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A paracentric inversion suppresses genetic recombination at the FON3 locus with breakpoints corresponding to sequence gaps on rice chromosome 11L

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Abstract

Paracentric inversion is known to inhibit genetic recombination between normal and inverted chromosomal segments in heterozygous arrangements. Insect inversion polymorphisms have been studied to reveal adaptive processes for maintaining genetic variation. We report the first paracentric inversion in rice (Oryza sativa), which was discovered in our effort to clone the floral organ number gene FON3. Recombination at the FON3 locus on the long arm of chromosome 11 was severely suppressed over a distance of more than 36 cM. An extensive screening among 8,242 F2 progeny failed to detect any recombinants. Cytological analysis revealed a loop-like structure on pachytene chromosomes, whereas FISH analysis showed the migration of a BAC clone from a distal location to a position closer to the centromere. Interestingly, the locations where the genetic recombination suppression began were coincided with the positions of two physical gaps on the chromosome 11, suggesting a correlation between the physical gaps, the inversion breakpoints. Transposons and retrotransposons, and tandemly arranged members of gene families were among the sequences immediately flanking the gaps. Taken together, we propose that the genetic suppression at the FON3 locus was caused by a paracentric inversion. The possible genetic mechanism causing such a spontaneous inversion was proposed.

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Acknowledgments

We thank the anonymous reviewers for detailed instructions for revising the manuscript and Charles H. Leseberg for his comments. The work was supported by grants from the Ministry of Science and Technology of China (2004CB117201) and the National Natural Science Foundation of China (30228022).

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Authors and Affiliations

  1. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Li Jiang, Wenli Zhang, Zhihui Xia, Guanghuai Jiang, Zhukuan Cheng, Lihuang Zhu, Long Mao & Wenxue Zhai

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100039, China

    Li Jiang & Wenli Zhang

  3. Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310006, China

    Qian Qian

  4. National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Aili Li & Long Mao

  5. Department of Biological Sciences, Northern Illinois University, DeKalb, IL, 60115, USA

    Long Mao

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  1. Li Jiang
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Correspondence to Long Mao or Wenxue Zhai.

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Communicated by R. Hagemann.

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Jiang, L., Zhang, W., Xia, Z. et al. A paracentric inversion suppresses genetic recombination at the FON3 locus with breakpoints corresponding to sequence gaps on rice chromosome 11L. Mol Genet Genomics 277, 263–272 (2007). https://doi.org/10.1007/s00438-006-0196-7

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