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Catfish CC chemokines: genomic clustering, duplications, and expression after bacterial infection with Edwardsiella ictaluri

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Abstract

Chemokines are a family of structurally related chemotactic cytokines that regulate the migration of leukocytes, under both physiological and inflammatory conditions. CC chemokines represent the largest subfamily of chemokines with 28 genes in mammals. Sequence conservation of chemokines between teleost fish and higher vertebrates is low and duplication and divergence may have occurred at a significantly faster rate than in other genes. One feature of CC chemokine genes known to be conserved is genomic clustering. CC chemokines are highly clustered within the genomes of human, mouse, and chicken. To exploit knowledge from comparative genome analysis between catfish and higher vertebrates, here we mapped to bacterial artificial chromosome (BAC) clones 26 previously identified catfish (Ictalurus sp.) chemokine cDNAs. Through a combination of hybridization and fluorescent fingerprinting, 18 fingerprinted contigs were assembled from BACs containing catfish CC chemokine genes. The catfish CC chemokine genes were found to be not only highly clustered in the catfish genome, but also extensively duplicated at various levels. Comparisons of the syntenic relationships of CC chemokines may help to explain the modes of duplication and divergence that resulted in the present repertoire of vertebrate CC chemokines. Here we have also analyzed the expression of the transcripts of the 26 catfish CC chemokines in head kidney and spleen in response to bacterial infection of Edwardsiella ictaluri, an economically devastating catfish pathogen. Such information should pinpoint research efforts on the CC chemokines most likely involved in inflammatory responses.

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Acknowledgements

This project was supported by a grant from USDA NRI Animal Genome Basic Genome Reagents and Tools Program (USDA/NRICGP 2003-35205-12827), in part by E-Institute of Shanghai Municipal Education Commission, Project number E03009, and in part by a Specific Cooperative Agreement with USDA ARS Aquatic Animal Health Laboratory under the Contract Number 58-6420-5-030. We thank Renee Beam, Karen Veverica, Esau Arana, and Randell Goodman for their excellence in the production and maintenance of fish used in this study and their assistance during challenge experiments.

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Authors and Affiliations

  1. The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures, Auburn University, 203 Swingle Hall, Auburn, AL, 36849, USA

    Eric Peatman, Baolong Bao, Xu Peng, Puttharat Baoprasertkul, Yolanda Brady & Zhanjiang Liu

  2. E-Institute of Shanghai Universities (EISU) Aquaculture Division and College of Aquatic Life Sciences and Technology, Shanghai Fisheries University, 200090, Shanghai, China

    Baolong Bao & Zhanjiang Liu

Authors
  1. Eric Peatman
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  2. Baolong Bao
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  3. Xu Peng
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  4. Puttharat Baoprasertkul
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  5. Yolanda Brady
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  6. Zhanjiang Liu
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Corresponding author

Correspondence to Zhanjiang Liu.

Additional information

Communicated by S. Hohmann

Eric Peatman and Baolong Bao contributed equally

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Peatman, E., Bao, B., Peng, X. et al. Catfish CC chemokines: genomic clustering, duplications, and expression after bacterial infection with Edwardsiella ictaluri . Mol Genet Genomics 275, 297–309 (2006). https://doi.org/10.1007/s00438-005-0081-9

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  • DOI: https://doi.org/10.1007/s00438-005-0081-9

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