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Multiple CC chemokines in channel catfish and blue catfish as revealed by analysis of expressed sequence tags

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Abstract

Chemokines represent a superfamily of chemotactic cytokines involved in recruitment, activation and adhesion of a variety of leukocyte types to inflammatory foci, as well as in the organization and maintenance of lymphoid organ architecture and in normal developmental processes. Nearly all chemokines have been identified in human and mouse, but only a handful of fish chemokines have been identified. Here we describe 14 distinct chemokines from channel catfish and blue catfish identified by analysis of 30,000 expressed sequence tags. Based on sequence analysis, sequence similarity, and the arrangement of the conserved cysteine residues, all 14 chemokines were identified as members of the CC subfamily. Phylogenetic analysis did not reveal clear evidence of orthology of the catfish and human or mouse chemokines. Similarity analysis indicated that nine of the 14 CC chemokines were identified for the first time in fish. The availability of this pool of catfish CC chemokines should facilitate rapid identification and phylogenetic analysis of CC chemokines from other fish and related species.

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Acknowledgements

This project was supported by a grant from USDA NRI Animal Genome Basic Genome Reagents and Tools Program (USDA/NRICGP 2003-35205-12827), and partially supported by a grant from Auburn University Competitive Biogrant Program, and a competitive grant from Auburn University AAES Foundation. We appreciate the support of Auburn University Department of Fisheries and Allied Aquacultures, College of Agriculture, and the Vice President for Research for their matching funds to USDA NRI Equipment Grants (98-35208-6540, 99-35208-8512). We would like to thank Dr John D. Hansen at Center of Marine Biotechnology at University of Maryland for his critical reading of the manuscript before submission to the journal.

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  1. The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, Auburn University, 203 Swingle Hall, Auburn, AL, 36849, USA

    Chongbo He, Eric Peatman, Puttharat Baoprasertkul, Huseyin Kucuktas & Zhanjiang Liu

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  1. Chongbo He
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  2. Eric Peatman
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  3. Puttharat Baoprasertkul
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Correspondence to Zhanjiang Liu.

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He, C., Peatman, E., Baoprasertkul, P. et al. Multiple CC chemokines in channel catfish and blue catfish as revealed by analysis of expressed sequence tags. Immunogenetics 56, 379–387 (2004). https://doi.org/10.1007/s00251-004-0697-7

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