Abstract
Expansins are thought to be key regulators of cell wall extension during plant growth. In this study, we isolated 18 expansin genes from wheat, nine of which encode α-expansins while the other nine code for β-expansins. The cysteine-rich and tryptophan-rich regions of the deduced amino acid sequences of all 18 expansins were highly conserved. Genomic sequences were obtained for 17 of the genes, and their intron patterns were determined. Four (A, C, D, E) of the six intron positions known in expansin genes from other species were found to be occupied in these wheat expansin genes. Five wheat expansin genes were mapped to chromosomes 1L, 2L, 5L and 6L respectively, by in silico and comparative mapping. The 18 wheat expansin genes were expressed in leaf, root and the developing seed. Moreover, it was demonstrated that four β-expansin genes were up-regulated in the internode tissue in F1 hybrids, suggesting that changes in the regulation of these genes in hybrid might contribute to the heterosis observed in internode length and plant height. We therefore conclude that expansins are encoded by a multigene family in wheat, and could play important roles in growth and development.
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References
Brummell DA, Harpster MH, Civello PM, Palys JM, Bennett AB, Dunsmuir P (1999) Modification of expansin protein abundance in tomato fruit alters softening and cell wall polymer metabolism during ripening. Plant Cell 11:2203–2216
Chen F, Dahal P, Bradford KJ (2001) Two tomato expansin genes show divergent expression and localization in embryos during seed development and germination. Plant Physiol 127:928–936
Cho HT, Kende H (1997) Expansins in deepwater rice internodes. Plant Physiol 113:1137–1143
Cosgrove DJ, Bedinger P, Durachko DM (1997) Plant biology group I allergens of grass pollen as cell wall-loosening agents. Proc Natl Acad Sci USA 94:6559–6564
Downes BP, Ryan SC, Crowell DN (2001) Expression and processing of a hormonally regulated β-expansin from soybean. Plant Physiol 126:244–252
Fleming AJ, McQueen-Mason SJ, Mandel T, Kuhlemeier C (1997) Induction of leaf primordia by the cell wall protein expansin. Science 276:1415–1418
Frugoli JA, McPeek MA, Thomas TL, McClung CR (1998) Intron loss and gain during evolution of the catalase gene family in angiosperms. Genetics 149:355–365
Gookin TE, Hunter DA, Reid MS (2003) Temporal analysis of alpha and beta-expansin expression during floral opening and senescence. Plant Sci 164:769–781
Harrison EP, McQueen-Mason SJ, Manning K (2001) Expression of six expansin genes in relation to extension activity in developing strawberry fruit. J Exp Bot 52:360:1437–1446
Lee Y, Kende H (2001) Expression of β-expansins is correlated with internodal elongation in deepwater rice. Plant Physiol 127:645–654
Lee Y, Choi D, Kende H (2001) Expansins: ever-expanding numbers and functions. Curr Opin Plant Biol 4:527–532
Lee DK, Ahn JH, Song SK, Choi YD, Lee JS (2003) Expression of an expansin gene is correlated with root elongation in soybean. Plant Physiol 131:985–997
Li Y, Darley CP, Ongaro VN, Fleming A, Schipper O, Baldauf SL, McQueen-Mason SJ (2002) Plant expansins are a complex multigene family with an ancient evolutionary origin. Plant Physiol 128:854–864
Li Y, Jones L, McQueen-Mason SJ (2003) Expansins and cell growth. Curr Opin Plant Biol 6:603–610
McQueen-Mason SJ, Durachko DM, Cosgrove DJ (1992) Two endogenous proteins that induce cell wall extension in plants. Plant Cell 4:1425–1433
Obenland DM, Crisosto CH, Rose JKC (2003) Expansin protein levels decline with the development of mealiness in peaches. Postharvest Biol Technol 29:11–18
Orford SJ, Timmis JN (1998) Specific expression of an expansin gene during elongation of cotton fibres. Biochim Biophys Acta 1398:342–346
Pien S, Wyrzykowska J, McQueen-Mason S, Smart C, Fleming A (2001) Local expression of expansin induces the entire process of leaf development and modifies leaf shape. Proc Natl Acad Sci USA 98:11812–11817
Reinhardt D, Wittwer F, Mandel T, Kuhlemeier C (1998) Localized upregulation of a new expansin gene predicts the site of leaf formation in the tomato meristem. Plant Cell 10:1427–1437
Rose JKC, Cosgrove DJ, Albersheim P, Darvill AG, Bennett AB (2000) Detection of expansin proteins and activity during tomato fruit ontogeny. Plant Physiol 123:1583–1592
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA space length polymophisms in barley: Mendelian inheritance,chromosomal locations and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
Sun QX, Wu LM, Ni ZF, Meng FM, Wang ZK, Lin Z (2004) Differential gene expression patterns in leaves between hybrids and their parental inbreds are correlated with heterosis in a wheat diallel cross. Plant Sci 166:651–657
Trivedi PK, Nath P (2004) MaExp1, an ethylene-induced expansin from ripening banana fruit. Plant Sci 167:1351–1358
Wu YJ, Meeley RB, Cosgrove DJ (2001) Analysis and expression of the α-expansin and β-expansin gene families in maize. Plant Physiol 126:222–232
Zhang NG, Hasenstein KH (2000) Distribution of expansins in graviresponding maize roots. Plant Cell Physiol 41:1305–1312
Acknowledgements
This work was financially supported by the State Key Basic Research and Development Plan of China (2001CB1088), National Science Found for Distinguished Young Scholars (39925026) and National Natural Science Foundation of China (30471078)
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Communicated by M.-A. Grandbastien
Z. Lin and Z. Ni contributed to this work equally
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Lin, Z., Ni, Z., Zhang, Y. et al. Isolation and characterization of 18 genes encoding α- and β-expansins in wheat (Triticum aestivum L.). Mol Genet Genomics 274, 548–556 (2005). https://doi.org/10.1007/s00438-005-0029-0
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DOI: https://doi.org/10.1007/s00438-005-0029-0