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Biochemical properties and vaccine effect of recombinant TPx-3 from Schistosoma japonicum

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Abstract

Thioredoxin peroxidases (TPxs) play an important role in maintaining redox homeostasis and in protecting organisms from the accumulation of toxic reactive oxygen species (ROS). In this study, we isolated the thioredoxin peroxidase-3 gene of Schistosoma japonicum, SjTPx-3. The open reading frame (ORF) of SjTPx-3 was 663 bp encoding 220 amino acids with a molecular weight of 24.99 kDa and an isoelectric point of 6.20. Quantitative real-time reverse transcription-polymerase chain reaction indicated that SjTPx-3 was expressed in all different stages of the parasites, with highest expression in 35-day-old worms. The ORF of SjTPx-3 was subcloned into pET-32a (+) vectors and expressed in Escherichia coli. Recombinant SjTPx-3 (rSjTPx-3) was expressed as a soluble protein with good antigenicity, as demonstrated by western blotting. Immunohistochemical analysis revealed that SjTPx-3 was mainly localized on the tegument of the parasites. Mice vaccinated with rSjTPx-3 had a 37.02% (P < 0.05) reduction in worm burden and 56.52% (P < 0.05) reduction in liver egg production compared with control, unvaccinated mice. Enzyme-linked immunosorbent assay analysis demonstrated that rSjTPx-3 could induce high levels of anti-rSjTPx-3-specific IgG, IgG1, and IgG2a antibodies. Characteristic Th1 and Th2 immune response cytokines were detected by flow cytometry and were increased by rSjTPx-3. Taken together, these results suggest that SjTPx-3 is an antioxidant enzyme responsible for protecting S. japonicum from oxidative stress. rSjTPx-3 may represent a potential vaccine candidate and/or new drug target for patients with schistosomiasis.

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Acknowledgements

We thank Yaojun Shi and Hao Li from Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, for technical assistance with parasite collection. This work was supported by the National Natural Science Foundation of China (Grant Nos. 31472188, 31172315, and 31402192), Key Projects in the National Science and Technology Pillar Program from the Ministry of Science and Technology (Grant No. 2015BAI09B04), and Fuzhiqiang subsidized by the program for leading personnel reserve of Minhang District, Shanghai (201442).

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Authors and Affiliations

  1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, 200241, People’s Republic of China

    Yanhui Han, Bin Zhao, Yang Hong, Hongxiao Han, Xiaodan Cao, Ke Lu, Jiaojiao Lin & Zhiqiang Fu

  2. College of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, 453003, People’s Republic of China

    Yanhui Han

  3. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan Province, 471023, People’s Republic of China

    Min Zhang

  4. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonose, Yangzhou, 225009, People’s Republic of China

    Jiaojiao Lin & Zhiqiang Fu

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  1. Yanhui Han
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Correspondence to Jiaojiao Lin or Zhiqiang Fu.

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Han, Y., Zhao, B., Zhang, M. et al. Biochemical properties and vaccine effect of recombinant TPx-3 from Schistosoma japonicum . Parasitol Res 116, 1361–1372 (2017). https://doi.org/10.1007/s00436-017-5415-0

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  • DOI: https://doi.org/10.1007/s00436-017-5415-0

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