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Complete mitochondrial genome analysis of Clinostomum complanatum and its comparison with selected digeneans

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Abstract

Clinostomum complanatum is an important trematode in fishes, birds of the family Ardeidae, and humans. Until now, limited knowledge is available regarding its molecular epidemiology, ecology, and phylogenetic study. Knowledge of the full mitochondrial genome of C. complanatum will provide important information for the study of epidemiology, biology, and genetic diversity of this fluke. In the present study, the complete mitochondrial genome of C. complanatum was sequenced and analyzed. The complete C. complanatum mitochondrial genome is 13,796 bp in length and contains 12 protein-coding genes, 22 tRNA genes, two rRNA genes, and one non-coding control region. All the 12 protein-coding genes are transcribed in the same direction and are AT-rich. Phylogenetic analysis based on concatenated amino acid sequences of the 12 protein-coding genes from C. complanatum and other selective digeneans showed that C. complanatum is in a separate branch, indicating C. complanatum has no closer relationship with any of the selected families. The complete mtDNA sequence of C. complanatum will increase our knowledge of mitochondrial genomics data and will also provide an important resource for studies of inter- and intra-species variation of flukes belonging to Clinostomidae.

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Acknowledgments

Sincere thanks to Professor Bang Shen for the comments on the manuscript. This work was supported in part by the “National Key Basic Research Program (973 Program) of China” (Grant No. 2015CB150300), the “Special Fund for Agro-scientific Research in the Public Interest” (Grant No. 201303037), and the “Fundamental Research Funds for the Central Universities” (Grant No. 2013PY059).

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    Authors and Affiliations

    1. State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, PR China

      Lu Chen, Xin Yang, Mudassar-Niaz Mughal & Rui Fang

    2. Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, PR China

      Lu Chen

    3. School of Urban Construction, Yangtze University, Jingzhou, 434023, Hubei, PR China

      Yan Feng

    4. Wuchang University of Technology, Wuhan, 430223, Hubei, PR China

      Hong-Mei Chen

    5. Hubei Provincial Center for Diseases Control and Prevention, Wuhan, 430079, Hubei, PR China

      Li-Xia Wang

    6. Hubei Entry-exit Inspection and Quarantine Bureau, Wuhan, 430022, Hubei, PR China

      Han-Li Feng

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    1. Lu Chen
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    Correspondence to Rui Fang.

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    Lu Chen and Yan Feng contributed equally to this work.

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    Chen, L., Feng, Y., Chen, HM. et al. Complete mitochondrial genome analysis of Clinostomum complanatum and its comparison with selected digeneans. Parasitol Res 115, 3249–3256 (2016). https://doi.org/10.1007/s00436-016-5102-6

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    • DOI: https://doi.org/10.1007/s00436-016-5102-6

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