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ATP synthase: an identified target gene of bantam in paired female Schistosoma japonicum

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Abstract

MicroRNAs (miRNAs) are a class of small non-coding RNAs that function in transcriptional and post-transcriptional regulation of gene expression. An increasing number of schistosome miRNAs have been identified and are expected possibly involved in differentiation, development, and metabolism. However, limited information is available concerning the target genes of schistosome miRNAs. In the present study, the key target genes of bantam, an abundant miRNA found in paired female Schistosoma japonicum, were predicted by bioinformatics analysis and Solexa technology. Luciferase reporter assay and bantam mimic assay were applied in combination to further verify the targets of bantam. Results showed that ATP synthase (CAX76793.1), one of the three selected predicted targets, was confirmed as the target of bantam; bantam mimic assay results also showed that the two other predicted targets, namely, ataxia telangiectasia mutated (ATM)-related (XP_002571630.1), and ribosomal protein L30 (CAX72575.1), were not confirmed as targets. This research proposed the design and significance of reasonable biological experiments that could be performed to identify miRNA target genes in schistosomes.

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Acknowledgments

We would like to thank Pan W. Q. for his valuable advice. We also appreciate the discussions and comments from Xiao S. H., Zhang Q. F., and Xu X. D. We thank Shenzhen Huada Gene Research Institute for Solexa analysis. This research was supported by the National Natural Science Foundation of China (Grant No. 81071383).

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Authors and Affiliations

  1. Tongji University School of Medicine, Shanghai, 200092, People’s Republic of China

    Jun Sun, Su-Wen Wang & Chen Li

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  1. Jun Sun
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  2. Su-Wen Wang
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  3. Chen Li
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Correspondence to Jun Sun.

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Table S1

A total of 232 up-regulated genes were predicted as target genes in 23DSI compared with 23SSI. (XLSX 35.2 kb)

Table S2

A total of 1619 down-regulated genes were predicted as target genes in 23DSI compared with 23SSI. (XLSX 167 kb)

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Sun, J., Wang, SW. & Li, C. ATP synthase: an identified target gene of bantam in paired female Schistosoma japonicum . Parasitol Res 114, 593–600 (2015). https://doi.org/10.1007/s00436-014-4221-1

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  • DOI: https://doi.org/10.1007/s00436-014-4221-1

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