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The occupation of intestinal epithelium by Trichinella spiralis in BALB/C mice is not associated with local manifestation of apoptosis related factors

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Abstract

Trichinella spiralis actively passes through the epithelial cells of the intestinal mucosa but morphologically, these cells do not manifest apparent damage. The possible activation of apoptotic mechanisms in the small intestine mucosa after infection with larvae and adults of Trichinella spiralis was explored by immunohistochemistry. Sporadic individual cells of normal intestinal epithelium showed activation of caspase-3, increased expression AIF, or Bax. The larval stage of intestinal trichinellosis was characterized by distortion of cells on the villus tips that were strongly reactive to caspase-3, Bax, and survivin antibodies. There was a transient loss of the survivin expression on the brush border of the epithelial cells at 15-h post infection, which reappeared on the fifth day. Bcl-2 changed its normal apical distribution and re-localized to the basal part of the epithelial cells. No significant changes of expression of the selected apoptosis-related proteins were observed in the intestinal epithelial cells immediately surrounding the worms. The presence of Trichinella affects intestinal epithelial cells, but unlike in muscle cells, invading them does not initiate apoptotic factors activation.

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Acknowledgments

The authors express sincere gratitude to Mrs. Emilia Klincová for her excellent technical work. This work was, in part, supported by grant VEGA 2/0011/12 and as the result of the project implementation: “TRANSMED 2”, ITMS: 26240120030, supported by the Research and Development Operational Programme funded by the European Regional Development Fund.

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Authors and Affiliations

  1. Department of Pathology, Comenius University in Bratislava, Bratislava, Slovakia

    Rositsa Milcheva, Pavol Janega & Pavel Babál

  2. Institute of Experimental Morphology, Pathology and Parasitology, Bulgarian Academy of Sciences, Sofia, Bulgaria

    Rositsa Milcheva & Svetlozara Petkova

  3. Institute of Parasitology, Slovak Academy of Sciences, Kosice, Slovakia

    Zuzana Hurniková

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  1. Rositsa Milcheva
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  2. Svetlozara Petkova
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  3. Zuzana Hurniková
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  4. Pavol Janega
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  5. Pavel Babál
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Correspondence to Pavel Babál.

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Milcheva, R., Petkova, S., Hurniková, Z. et al. The occupation of intestinal epithelium by Trichinella spiralis in BALB/C mice is not associated with local manifestation of apoptosis related factors. Parasitol Res 112, 3917–3924 (2013). https://doi.org/10.1007/s00436-013-3582-1

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