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Phylogenetic study of Setaria cervi based on mitochondrial cox1 gene sequences

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Abstract

The objective of the present study was to examine the phylogenetic position of Setaria cervi based on sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. A fragment of the cox1 gene from two morphologically identified S. cervi collected from red deer (Cervus elaphus) from Italy were amplified, sequenced, and compared with corresponding sequences of other filarioid nematode species. Phylogenetic studies using Bayesian analysis revealed S. cervi as monophyletic with other Setaria species, confirming S. cervi as a member of the Setaria genus. S. cervi appeared to be sister species to Setaria labiatopapillosa and Setaria digitata. Setaria tundra and Setaria equina, the other two Setaria species presented in the Italian fauna, formed a sister group to the clade consisting of S. cervi, S. labiatopapillosa, and S. digitata. In addition to phylogenetic clarification, our study is the first molecular identification of S. cervi, which may be useful for further molecular identification and differentiation of this filarial worm from other filarioid nematode species, especially in the earlier developmental stages of its life cycle.

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Acknowledgments

The research was supported by RNM-6400, Projecto de Excelencia (Junta de Andalucia, Spain). XQZ is supported by the State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (Grant Nos. SKLVEB2009KFKT014 and SKLVEB2010KFKT010), and the Yunnan Provincial Program for Introducing High-level Scientists (Grant No. 2009CI125). The experiments comply with the current laws of the country in which the experiments were performed.

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Authors and Affiliations

  1. Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Università degli Studi di Torino, Via Leonardo da Vinci 44, 10095, Grugliasco, Italy

    Samer Alasaad & Luca Rossi

  2. Istituto “G. Caporale”, Campo Boario, 64100, Teramo, Italy

    Ilaria Pascucci

  3. Estación Biológica de Doñana, Consejo Superior de Investigaciones Científicas (CSIC), Avda. Americo Vespucio s/n, 41092, Seville, Spain

    Samer Alasaad, Michael J. Jowers & Ramón C. Soriguer

  4. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu Province, 730046, People’s Republic of China

    Xing-Quan Zhu

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  1. Samer Alasaad
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  2. Ilaria Pascucci
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  3. Michael J. Jowers
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  4. Ramón C. Soriguer
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  5. Xing-Quan Zhu
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  6. Luca Rossi
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Correspondence to Samer Alasaad.

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Alasaad, S., Pascucci, I., Jowers, M.J. et al. Phylogenetic study of Setaria cervi based on mitochondrial cox1 gene sequences. Parasitol Res 110, 281–285 (2012). https://doi.org/10.1007/s00436-011-2486-1

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  • DOI: https://doi.org/10.1007/s00436-011-2486-1

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