Abstract
After the introduction of bluetongue in northern Europe in 2006, populations of Culicoides have been monitored in many European countries. Large quantities of Culicoides specimens shall be determined to species, and it is thus important to find reliable morphological characters that are visualized in a stereomicroscope. Culicoides obsoletus, Culicoides scoticus, Culicoides chiopterus, and Culicoides dewulfi all belonging to subgenus Avaritia are common in collections in northern Europe. C. obsoletus and C. scoticus often make up more than 50% of the total catch of biting midges. Separation of the females of the four species by morphological characters has frequently been questioned, and in many cases, female specimens are grouped as an entity in veterinary and ecological studies. We show how it is possible using a stereomicroscope to separate the females of the four species by combining the shape of the third segment of the maxillary palp and the number and location of hairs on the first abdominal tergit. Validation of the quick stereomicroscope identification method was achieved by morphometric measurements and a molecular marker. In all cases, both methods verified the quick morphological species identification of the Obsoletus group females. In conclusion, the females of all four species of the Obsoletus group can be separated by a quick morphological method under the stereomicroscope.
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References
Augot D, Sauvage F, Jouet D, Simphal E, Veuille M, Couloux A, Kaltenbach ML, Depaquit J (2010) Discrimination of Culicoides obsoletus and C. scoticus, potential bluetongue vectors, by morphometrical and mitochondrial cytochrome oxidase subunit I analysis. Infect Genet Evol 10:629–637
Baldet T, Delecolle JC, Cetre-Sossah C, Mathieu B, Meiswinkel R, Gerbier G (2008) Indoor activity of Culicoides associated with livestock in the bluetongue virus (BTV) affected region of Northern France during autumn 2006. Prev Vet Med 87:84–97
Baylis M, Mellor PS, Wittmann EJ, Rogers DJ (2001) Prediction of areas around the Mediterranean at risk of bluetongue by modelling the distribution of its vector using satellite imaging. Vet Rec 149:639–643
Campbell JA, Pelham-Clinton EC (1960) A taxonomic review of the British species of Culicoides Latreille (Diptera, Ceratopogonidae). Proc R Soc Edinb B67:181–302
Carpenter S, Lunt HL, Arav D, Venter GJ, Mellor PS (2006) Oral susceptibility to bluetongue virus of Culicoides (Diptera: Ceratopogonidae) from the United Kingdom. J Med Entomol 43:73–78
Cetre-Sossah C, Baldet T, Delecolle JC, Mathieu B, Perrin A, Grillet C, Albina E (2004) Molecular detection of Culicoides spp. and Culicoides imicola, the principal vector of bluetongue (BT) and African horse sickness (AHS) in Africa and Europe. Vet Res 35:325–337
Cetre-Sossah C, Mathieu B, Setier-Rio ML, Grillet C, Baldet T, Delecolle JC, Albina E (2008) Development and evaluation of a real-time quantitative PCR assay for Culicoides imicola, one of the main vectors of bluetongue (BT) and African horse sickness (AHS) in Africa and Europe. Res Vet Sci 85:372–382
Dallas JF, Cruickshank RH, Linton YM, Nolan DV, Patakakis M, Braverman Y, Capela M, Pena I, Mesiwinkel R, Ortega R, Baylis MD, Mellor PS, Mordue Lunz AJ (2003) Phylogenetic status and matrilineal structure of the biting midge, Culicoides imicola, in Portugal, Rhodes and Israel. Med Vet Entomol 17:379–387
De Liberato C, Scavia G, Lorenzetti R, Scaramozzino P, Amaddeo D, Cardeti G, Scicluna M, Ferrari G, Autorino GL (2005) Identification of Culicoides obsoletus (Diptera: Ceratopogonidae) as a vector of bluetongue virus in central Italy. Vet Rec 156:301–304
Dzhafarov SH (1976) Biting midges (Diptera, Heleidae) of Transcaucasus (Morphology, biology, ecology, geographical distribution, and harmfulness, control, fauna of the genera Culicoides, Leptoconops and Lasiohelea). Publishing House of the Academy of Science of Azerbaijan SSR.Translated for the Agricultural Research Service, U.S. Department of Agriculture, and the National Science Foundation Washington, D.C. by Franklin Book Programs. Inc., Cairo, 1976
Garros C, Mathieu B, Balenghien T, Cêtre-Sossah C, Delécolle JC (2010) Suggesting synonymies? Comments on Kiehl et al. (2009) "The European vectors of bluetongue virus: are there species complexes, single species or races in Culicoides obsoletus and C. pulicaris detectable by sequencing ITS-1, ITS-2 and 18S-rDNA.". Parasitol Res 107:731–734
Gloster J, Burgin L, Witham C, Athanassiadou M, Mellor PS (2008) Bluetongue in the United Kingdom and Northern Europe in 2007 and key issues for 2008. Vet Rec 162:298–302
Glukhova VM (2005) Culicoides (Diptera: Ceratopogonidae) of Russian and adjacent lands. Int J Dipterol Res 16:3–75
Gomulski LM, Meiswinkel R, Delecolle JC, Goffredo M, Gasperi G (2005) Phylogenetic relationships of the subgenus Avaritia Fox, 1955 including Culicoides obsoletus (Diptera, Ceratopogonidae) in Italy based on internal transcribed spacer 2 ribosomal DNA sequences. Syst Entomol 30:619–631
Gomulski LM, Meiswinkel R, Delecolle JC, Goffredo M, Gasperi G (2006) Phylogeny of the subgenus Culicoides and related species in Italy, inferred from internal transcribed spacer 2 ribosomal DNA sequences. Med Vet Entomol 20:229–238
Huldén L (2006) Culicoides-arterne (Diptera, Ceratopogonidae) i Finland och grannländerne. Department of Applied Biology Helsinki University. Helsinki, Finland. Report: 1–19.
Kiehl E, Walldorf V, Klimpel S, Al-Quraishy S, Mehlhorn H (2009) The European vectors of bluetongue virus: are there species complexes, single species or races in Culicoides obsoletus and C. pulicaris detectable by sequencing ITS-1, ITS-2 and 18S-rDNA. Parasitol Res 105:331–336
Kremer M (1965) Contribution à l`étude du genre Culicoides Latreille particulièrement en France. Encycl Ent A 39:1–299
Linton YM, Mordue Lunz AJ, Cruickshank RH, Meiswinkel R, Mellor PS, Dallas JF (2002) Phylogenetic analysis of the mitochondrial cytochrome oxidase subunit I gene of five species of the Culicoides imicola species complex. Med Vet Entomol 16:139–146
Mathieu B, Perrin A, Baldet T, Delecolle JC, Albina E, Cetre-Sossah C (2007) Molecular identification of western European species of Obsoletus complex (Diptera: Ceratopogonidae) by an internal transcribed spacer-1 rDNA multiplex polymerase chain reaction assay. J Med Entomol 44:1019–1025
Mehlhorn H, Walldorf V, Klimpel S, Schmahl G (2008) Outbreak of bluetongue disease (BTD) in Germany and the danger for Europe. Parasitol Res 103:S79–S86
Meiswinkel R, van Rijn P, Leijs P, Goffredo M (2007) Potential new Culicoides vector of bluetongue virus in northern Europe. Vet Rec 161:564–565
Mellor PS, Wittmann EJ (2002) Bluetongue virus in the Mediterranean Basin 1998–2001. Vet J 164:20–37
Nielsen SA, Nielsen BO, Axelsen JA, Fotel FL (1998) Forurening og formering af mitter i Egeløkke Lung. Larvetæthed og aktiviteter af voksne mitter. Report 423, 1–54. Danish EPA. Copenhagen, Denmark
Nielsen SA, Nielsen BO, Chirico J (2010) Monitoring of biting midges (Diptera: Culicoides Latreille) on farms in Sweden during the emergence of the 2008 epidemic of bluetongue. Parasitol Res 106:1197–1203
Nolan DV, Carpenter S, Barber J, Mellor PS, Dallas JF, Mordue Luntz AJ, Piertney SB (2007) Rapid diagnostic PCR assays for members of the Culicoides obsoletus and Culicoides pulicaris species complexes, implicated vectors of bluetongue virus in Europe. Vet Microbiol 124:82–94
Nolan DV, Dallas JF, Piertney SB, Mordue Luntz AJ (2008) Incursion and range expansion in the bluetongue vector Culicoides imicola in the Mediterranean basin: a phylogeographic analysis. Med Vet Entomol 22:340–351
Pages N, Monteys VSI (2005) Differentiation of Culicoides obsoletus and Culicoides scoticus (Diptera: Ceratopogonidae) based on mitochondrial cytochrome oxidase subunit I. J Med Entomol 42:1026–1034
Perrin A, Cetre-Sossah C, Mathieu B, Baldet T, Delecolle JC, Albina E (2006) Phylogenetic analysis of Culicoides species from France based on nuclear ITS1-rDNA sequences. Med Vet Entomol 20:219–228
Purse BV, Mellor PS, Rogers DJ, Samuel AR, Mertens PPC, Baylis M (2005) Climate change and the recent emergence of bluetongue in Europe. Nature Rev Microbiol 3:171–181
Purse BV, Nedelchev N, Georgiev G, Veleva E, Boorman J, Denison E, Veronesi E, Carpenter S, Baylis M, Mellor PS (2006) Spatial and temporal distribution of bluetongue and its Culicoides vectors in Bulgaria. Med Vet Entomol 20:335–344
Remm H (1988) Ceratopogonidae. [in:] Á. Soos, L. Papp (eds.) Catalogue of Palaearctic Diptera 3: 11–110
Root FM, Hoffman WA (1937) The North American species of Culicoides. Amer J Hyg 25:150–176
Saegerman C, Berkvens D, Mellor PS (2008) Bluetongue epidemiology in the European Union. Emerg Infect Dis 14:539–544
Savini G, Goffredo M, Monaco F, Di Gennaro A, Cafiero MA, Baldi L, de Santis P, Meiswinkel R, Caporale V (2005) Bluetongue virus isolations from midges belonging to the Obsoletus complex (Culicoides, Diptera: Ceratopogonidae) in Italy. Vet Rec 157:133–139
Schwenkenbecher JM, Mordue Lunz AJ, Piertney SB (2009) Phylogenetic analysis indicates that Culicoides dewulfi should not be considered part of the Culicoides obsoletus complex. Bull Entomol Res 46:610–614
Wilson A, Mellor PS (2008) Bluetongue in Europe: vectors, epidemiology and climate change. Parasitol Res 103:S69–S77
Acknowledgments
We are indebted to laboratory engineer Katrine Bøg for editing the pictures. The project was supported by the Danish Ministry of Food, Agriculture and Fisheries.
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Nielsen, S.A., Kristensen, M. Morphological and molecular identification of species of the Obsoletus group (Diptera: Ceratopogonidae) in Scandinavia. Parasitol Res 109, 1133–1141 (2011). https://doi.org/10.1007/s00436-011-2357-9
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DOI: https://doi.org/10.1007/s00436-011-2357-9