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Comparative genotyping of Echinococcus granulosus infecting buffalo in Iran using cox1 gene

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Abstract

Twenty-five isolates of Echinococcus granulosus protoscoleces from hydatid cysts were collected from 25 buffaloes in five different provinces of Iran. DNA was extracted from protoscoleces and used for phylogenetical analysis. For this aim, the DNA was amplified using specific primers derived from cox1 gene and, subsequently, the corresponding polymerase chain reaction products were sequenced. The sequence analysis showed five genotypes within the samples. Twenty-three isolates were identified as G1 and under grouped as separate genotypes: G1α, G1β, G1γ and G1δ, and two isolates were identified as G3 strain in Iran. G3 strain has not been already reported in buffaloes in Iran. The nucleotide and amino acid sequences of cox1 gene from corresponding isolates were documented in GenBank under accession numbers: HM130574, HM130575, HM130576, HM130577, HM130578, HM130579, HM130580, HM130581, HM130582, HM130583, HM130584, HM130585, HM130586, HM130587, HM130588, HM130589, HM130590, HM130591, HM130592, HM130593, HM130594, HM130595, HM130596, HM130597 and HM 130598.

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Acknowledgements

We thank Mrs. Narges Aminian for the technical help. We also thank Dr. Nurolahi, Dr. Razbani, Dr. Behruzi and Dr. Alavi from the Veterinary Organization of Iran for their help in collecting the samples from different slaughterhouses.

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Authors and Affiliations

  1. Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

    Arash Amin Pour, Seyed Hossein Hosseini & Parviz Shayan

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  1. Arash Amin Pour
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  2. Seyed Hossein Hosseini
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  3. Parviz Shayan
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Correspondence to Parviz Shayan.

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Amin Pour, A., Hosseini, S.H. & Shayan, P. Comparative genotyping of Echinococcus granulosus infecting buffalo in Iran using cox1 gene. Parasitol Res 108, 1229–1234 (2011). https://doi.org/10.1007/s00436-010-2170-x

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  • DOI: https://doi.org/10.1007/s00436-010-2170-x

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