Abstract
Leishmaniasis causes considerable mortality throughout the world, affecting more than 12 million people. Cymbopogon citratus (DC) Stapf, Family Poaceae, is a widely used herb in tropical countries and is also known as a source of ethnomedicines. In this study, the inhibitory effect and the morphological and ultrastructural alterations on Leishmania amazonensis by the essential oil (EO) of C. citratus and its main constituent, citral, were evaluated. The results showed that the antiproliferative activity of EO on promastigotes and axenic amastigotes, and intracellular amastigote forms of L. amazonensis was significantly better than citral, and indicated a dose-dependent effect. Neither compound showed a cytotoxic effect on macrophage strain J774G8. The promastigote forms of L. amazonensis underwent remarkable morphological and ultrastructural alterations compared with untreated cultures. These alterations were visible by light, scanning, and transmission electron microscopy of promastigotes treated with EO and citral at concentrations corresponding to the IC50 (1.7 and 8.0 µg/ml) and IC90 (3.2 and 25 µg/ml), respectively, after 72 h of incubation. This study revealed that citral-rich essential oil from C. citratus has promising antileishmanial properties, and is a good candidate for further research to develop a new anti-protozoan drug.
Similar content being viewed by others
References
Adams RP (2007) Identification of essential oil components by gas chromatography/mass spectrometry; 4th edn. Allured, Carol Stream, Illinois, p 804
Adegoke GO, Odesola BA (1996) Storage of maize and cowpea and inhibition of microbial agents of biodeterioration using the powder and essential oil of lemon grass (Cymbopogon citratus). Int Biodeterior Biodegrad 37:81–84
Anthony JP, Fyfe L, Smith H (2005) Plant active components—a resource for antiparasitic agents? Trends Parasitol 21:462–468
Barbosa LCA, Pereira UA, Martinazzo AP, Maltha CRA, Teixeira RR, Melo EC (2008) Evaluation of the chemical composition of Brazilian commercial Cymbopogon citratus (D.C.) stapf samples. Molecules 13:1864–1874
Blanco MM, Costa CARA, Freire AO, Santos JG Jr, Costa M (2009) Neurobehavioral effect of essential oil of Cymbopogon citratus in mice. Phytomedicine 16(2–3):265–270
Calixto JB (2000) Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents). Braz J Med Biol Res 33:179–189
Camacho MDR, Phillipson JD, Croft SL, Solis PN, Marshall SJ, Ghazanfar SA (2003) Screening of plant extracts for antiprotozoal and cytotoxic activities. J Ethnopharmacol 89:185–191
Carvalho PB, Ferreira EI (2001) Leishmaniasis phytotherapy. Nature's leadership against an ancient disease. Fitoterapia 72:599–618
Chen M, Christensen SB, Blom J, Lemmich E, Nadelmann L, Fich K, Theander TG, Kharazmi A (1993) Licochalcone A, a novel antiparasitic agent with potent activity against human pathogenic protozoan species of Leishmania. Antimicrob Agents Chemother 37:2550–2556
Cunningham AC (2002) Parasitic adaptive mechanisms in infection by Leishmania. Exp Mol Pathol 72:132–141
Desjeux P (2004) Leishmaniasis: current situation and new perspectives. Comp Immunol Microbiol Infect Dis 27:305–318
Hernández NE, Tereschuk ML, Abdala LR (2000) Antimicrobial activity of flavonoids in medicinal plants from Tafí del Valle (Tucumán, Argetina). J Ethnopharmacol 73:317–322
Kanko C, Sawaliho E, Kone S, Koukoua G, N'guessan YT (2004) Étude des propriétés physico-chimiques des huiles essentielles de Lippia multiflora, Cymbopogon citratus, Cymbopogon nardus, Cymbopogon giganteus. C R Chim 7:1039–1049
Lewinsohn E, Dudai N, Tadmor Y, Katzir I, Ravid U, Putievsky E, Joel DM (1998) Histochemical localization of citral accumulation in lemongrass leaves (Cympobogon citratus (DC.) Stapf., Poaceae). An Bot 81:35–39
Luize PS, Tiuman TS, Morello LG, Maza PK, Ueda-Nakamura T, Filho BPD, Cortez DAG, Mello JCP, Nakamura CV (2005) Effects of medicinal plant extracts on growth of Leishmania (L.) amazonensis and Trypanosoma cruzi. Braz J Pharm Sci 41:85–94
McConville MJ, Handman E (2007) The molecular basis of Leishmania pathogenesis. Int J Parasitol 37:1047–1051
Melo SF, Soares SF, Costa RF, Silva CR, Oliveira MBN, Bezerra RJAC, Caldeira-de-Araujo A, Bernardo-Filho M (2001) Effect of the Cymbopogon citratus, Maytenus ilicifolia and Baccharis genistelloides extracts against the stannous chloride oxidative damage in Escherichia coli. Mutat Res 496:33–38
Pedroso RB, Ueda-Nakamura T, Dias Filho BP, Cortez DAG, Cortez LER, Morgado-Díaz JA, Nakamura CV (2006) Biological activities of essential oil obtained from Cymbopogon citratus on Crithidia deanei. Acta Protozool 45:231–240
Puatanachokchai R, Kishida H, Denda A, Murata N, Konishi Y, Vinitketkumnuen U, Nakae D (2002) Inhibitory effects of lemon grass (Cymbopogon citratus, Stapf) extract on the early phase of hepatocarcinogenesis after initiation with diethylnitrosamine in male Fischer 344 rats. Cancer Lett 183:9–15
Rates SMK (2001) Plants as source of drugs. Toxicon 39:603–316
Rauber CS, Guterres SS, Schapoval EES (2005) LC determination of citral in Cymbopogon citratus volatile oil. J Pharm Biomed Anal 37:597–601
Rodrigues JCF, Attias M, Rodriguez C, Urbina JA, Souza W (2002) Ultrastructural and biochemical alterations induced by 22, 26-azasterol, a Δ24(25)-sterol methyltransferase inhibitor, on promastigote and amastigote forms of Leishmania amazonensis. Antimicrob Agents Chemother 46:487–499
Santa-Rita RM, Henriques-Pons A, Barbosa HS, Castro SL (2004) Effect of the lysophospholipid analogues edelfosine, ilmofosine and miltefosine against Leishmania amazonensis. J Antimicrob Chemother 54:704–710
Santoro GF, Cardoso MG, Guimarães LGL, Freire JM, Soares MJ (2007) Anti-proliferative effect of the essential oil of Cymbopogon citratus (DC) Stapf (lemongrass) on intracellular amastigotes, bloodstream trypomastigotes and culture epimastigotes of Trypanosoma cruzi (Protozoa: Kinetoplastida). Parasitol 134:1649–1656
Ueda-Nakamura T, Attias M, Souza W (2001) Megasome biogenesis in Leishmania amazonensis: a morphometric and cytochemical study. Parasitol Res 87:89–97
Viana GSB, Vale TG, Pinho RSN, Matos FJA (2000) Antinociceptive effect of the essential oil from Cymbopogon citratus in mice. J Ethnopharmacol 70:323–327
WHO - WORLD HEALTH ORGANIZATION. Leishmaniasis: disease burden and epidemiological trends. Special Programme for Research and Training in Tropical Diseases. Geneva: WHO, 2002. Online:http://www.who.int/tdr/diseases/leish/files/direction.pdf. Accessed 11 december 2007
Acknowledgments
This study was supported through grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq, Capacitação de Aperfeiçoamento de Pessoal de Nível Superior—CAPES, Financiadora de Estudos e Projetos—FINEP, and Programa de Pós-graduação em Ciências Farmacêuticas da Universidade Estadual de Maringá.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Santin, M.R., dos Santos, A.O., Nakamura, C.V. et al. In vitro activity of the essential oil of Cymbopogon citratus and its major component (citral) on Leishmania amazonensis . Parasitol Res 105, 1489–1496 (2009). https://doi.org/10.1007/s00436-009-1578-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00436-009-1578-7