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Prevalence and distribution of Cryptosporidium spp. in dairy cattle in Heilongjiang Province, China

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Abstract

Few data are available on the molecular characterization of Cryptosporidium spp. in cattle in China. In the present study, a total of 507 fecal specimens from six dairy farms in Heilongjiang Province were examined for Cryptosporidium spp. by light microscopy of concentrates from the formalin-ethyl acetate sedimentation method (for less than 2-month-old calves) or Sheather’s floatation method (more than 3-month-old dairy cattle). Twenty-seven post-weaned calves on five farms were positive for Cryptosporidium oocysts. PCR and DNA sequence analysis of the 18S rRNA, actin, and 70 kDa heat shock protein genes identified Cryptosporidium andersoni and Cryptosporidium. ryanae, with C. andersoni as the dominant species (26 out of 27). In comparison with other regions of the world, the distribution of Cryptosporidium species in the areas appears to be unique.

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Acknowledgement

This work was supported in part by the Natural Science Foundation of Heilongjiang Province (grant D200628) and the Heilongjiang Province Education Bureau (grant 11521082), China. We thank YL Jin at Heilongjiang Animal Health Inspection Institute for help in providing the specimens.

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Authors and Affiliations

  1. Department of Parasitology, Harbin Medical University, 194 Xuefu Road, Harbin, Heilongjiang, 150081, China

    Aiqin Liu, Yihong Li, Jing Shu, Weizhe Zhang & Hong Ling

  2. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, 450002, China

    Rongjun Wang & Longxian Zhang

  3. School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China

    Yaoyu Feng

  4. Division of Parasitic Diseases, Center for Disease Control and Prevention, Atlanta, GA, 30341, USA

    Lihua Xiao

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  1. Aiqin Liu
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Correspondence to Hong Ling.

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Liu, A., Wang, R., Li, Y. et al. Prevalence and distribution of Cryptosporidium spp. in dairy cattle in Heilongjiang Province, China. Parasitol Res 105, 797–802 (2009). https://doi.org/10.1007/s00436-009-1457-2

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  • DOI: https://doi.org/10.1007/s00436-009-1457-2

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