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Phylogenetic relationships of Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) within the order Spirurida inferred using mitochondrial cytochrome c oxidase subunit 1 (cox1) gene analysis

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Abstract

The present study investigated genetic variability within a population of Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) affecting horses in an endemic area of central Italy using polymerase chain reaction (PCR)-coupled sequencing of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1). No different cox1 sequences were detected in any of the H. muscae individual, while two haplotypes representing H. microstoma individuals differed for one substitution. The pairwise distance between the H. muscae and H. microstoma was 11%, coding for five amino acid changes. The sequence of an informative region within the cox1 gene of H. microstoma and H. muscae was analyzed by Maximum Likelihood and Bayesian phylogenetic methods using available mitochondrial sequences spirurid taxa belonging to Filarioidea, Thelazioidea, and Habronematoidea. Phylogenetic analysis supported the split of the tree into two sister spirurid groups, Habronematoidea and Filarioidea + Thelazioidea. The phylogenetic and evolutionary implications of Habronema with Filaroidea and Thelazioidea are discussed.

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Author notes

  1. Raffaella Iorio

    Present address: Faculty of Agronomy, Teramo, Italy

Authors and Affiliations

  1. Department of Comparative Biomedical Sciences, Faculty of Veterinary Medicine, University of Teramo, Piazza Aldo Moro 45, 64100, Teramo, Italy

    Raffaella Iorio, Barbara Paoletti & Donato Traversa

  2. Faculty of Veterinary Science, University of Sydney, New South Wales, Australia

    Jan Šlapeta

  3. Department of Veterinary Public Health and Animal Sciences, Faculty of Veterinary Medicine, Valenzano, Bari, Italy

    Domenico Otranto

  4. Department of Production Science, Engineering, Mechanics and Economy, Faculty of Agronomy, University of Foggia, Foggia, Italy

    Annunziata Giangaspero

Authors
  1. Raffaella Iorio
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  2. Jan Šlapeta
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  3. Domenico Otranto
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  4. Barbara Paoletti
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  5. Annunziata Giangaspero
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  6. Donato Traversa
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Correspondence to Donato Traversa.

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Iorio, R., Šlapeta, J., Otranto, D. et al. Phylogenetic relationships of Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) within the order Spirurida inferred using mitochondrial cytochrome c oxidase subunit 1 (cox1) gene analysis. Parasitol Res 104, 979–984 (2009). https://doi.org/10.1007/s00436-008-1276-x

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  • DOI: https://doi.org/10.1007/s00436-008-1276-x

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