Abstract
The aim of this study is to evaluate cellular interaction between free-living amoebae Naegleria fowleri strains and mammalian target cells in vitro. Two Thai strains of N. fowleri; Khon Kaen strain from the environment and Siriraj strain from the patient’s cerebrospinal fluid and the Center of Disease Control VO 3081 strain from Atlanta (US) were studied. Human neuroblastoma (SK-N-MC) and African Green monkey Kidney (Vero) cells were used as target cells. Each cell line was inoculated with each strain of N. fowleri at a ratio of 1:1 and observed for 7 days. The uninoculated target cells and each strain of N. fowleri were used as control. The numbers of the challenged and unchallenged cells as well as the free-living amoebae were counted three times by trypan blue exclusion method. The inoculation began when the amoebae attached to the cell membrane and ingested the target cells. In this study, extensive cytopathogenesis with many floating inoculated cells and abundant number of amoebae were observed. The destruction pattern of both inoculated SK-N-MC and Vero target cells were similar. Interestingly, SK-N-MC was more susceptible to N. fowleri strains than the Vero cell. In addition, N. fowleri Siriraj strain showed the highest destruction pattern for each target cell. Our findings suggest that the SK-N-MC should be used as a base model for studying the neuropathogenesis in primary amoebic meningoencephalitis patients.
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References
Biedler JL, Helson L, Spengler BA (1973) Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture. Cancer Res 3:2643–2652
De Jonckheere JF (2002) A century of research on the amoeboflagellate genus Naegleria. Acta Protozool 41:309–342
Fulford DE, Marciano-Cabral F (1986) Cytopathic activity of Naegleria fowleri cell-free extract. J Protozool 33:498–450
Jeong SR, Lee SC, Song KJ, Park S, Kim K, Kwon MH, Im Ki, Shin HJ (2005) Expression on the nfa1 gene cloned from pathogenic Naegleria fowleri in nonpathogenic N. gluberi enhances cytotoxicity against CHO target cells in vitro. Infect Immun 73:4098–4105
John DT, John RA (1994a) Use of Vero-cell culture to assess cytopathogenicity of Naegleria species. Proc Okla Acad 74:17–20
John DT, John RA (1994b) Enhancement of virulence of Naegleria fowleri by growth in Vero-cell culture. J Parasitol 80:149–150
Marciano-Cabral F, John DT (1983) Cytopathogenicity of Naegleria fowleri for rat neuroblastoma cell cultures: scanning electron microscopy study. Infect Immun 40:1214–1217
Nelson EC, Jones MM (1970) Culture isolation of agents of primary amebic meningoencephalitis. J Parasitol 56:248
Song KJ, Song KH, Na BK, Kim JH,Kwon D, Park S, Pak JH, Im KII, Shin HJ (2007) Molecular cloning and characterization of a cytosolic heat shock protein 70 from Naegleria fowleri. Parasitol Res 100:1083–1089 DOI 1007/s00436–006–0404–8
Tiewcharoen S, Komalamisara N, Junnu V (2004) Naegleria fowleri in Thailand 2003. Southeast Asian J Trop Med Public Health 35:24–31
Yum H-C, Park S-J, Kong H-H, Chung D-I (2002) Isolation of genes induced in Naegleria fowleri during mouse brain passage. Eur J Protisol 38:105–111
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This work was partially funded by the Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand in 2005–2006.
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Tiewcharoen, S., Malainual, N., Junnu, V. et al. Cytopathogenesis of Naegleria fowleri Thai strains for cultured human neuroblastoma cells. Parasitol Res 102, 997–1000 (2008). https://doi.org/10.1007/s00436-007-0866-3
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DOI: https://doi.org/10.1007/s00436-007-0866-3