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Genotype of Giardia intestinalis isolates from children and dogs and its relationship to host origin

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Abstract

The presence of human Giardia in several animals suggests a zoonotic transmission. We studied G. Intestinalis isolates obtained from: children with diarrhea (n=6), asymptomatic children (n=7), axenic cultures (n=7) and dogs (n=11). The sequence corresponding to 16 S rRNA was amplified by PCR, sequenced and compared with genotypes A, B and Dog sequences reported in the Gene Bank database. Results show that 9/20 (45%) of children isolates belonged to genotype A and 11/20 (55%) showed some variable sites, allowing classification in three arbitrary clusters: A1, A2 and A3. In adition 7/11 (63%) of dog isolates were genotype A, including those dogs that lived in the same locality as the children lived, while 4/11 (37%) belonged to an arbitrary A4 cluster living in a different locality. In this study, genotype A was associated with samples from children and dogs, and, therefore, we could infer zoonotic transmission as a way of getting the disease.

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Authors and Affiliations

  1. Laboratory of parasitology Research, Hospital Infantil de México Federico Gómez, Dr. Márquez# 162, Col. Doctores, 06720 DF, Mexico

    Leticia Eligio-García, Adrian Cortes-Campos & Enedina Jiménez-Cardoso

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  1. Leticia Eligio-García
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  2. Adrian Cortes-Campos
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  3. Enedina Jiménez-Cardoso
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Correspondence to Enedina Jiménez-Cardoso.

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Eligio-García, L., Cortes-Campos, A. & Jiménez-Cardoso, E. Genotype of Giardia intestinalis isolates from children and dogs and its relationship to host origin. Parasitol Res 97, 1–6 (2005). https://doi.org/10.1007/s00436-005-1368-9

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  • DOI: https://doi.org/10.1007/s00436-005-1368-9

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