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The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens

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Abstract

When in vitro cultivation was used as the ‘gold standard’ for the detection of Blastocystis hominis in stool specimens, simple smear and trichrome staining showed sensitivities of 16.7% and 40.2% and specificities of 94% and 80.4%, respectively. In vitro cultivation also enhanced PCR amplification for the detection of B. hominis in stool specimens. Our data show the usefulness of in vitro cultivation for the detection and molecular study of B. hominis in stool specimens.

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References

  • Ashford RW, Atkinson EA (1992) Epidemiology of Blastocystis hominis infection in Papua New Guinea: age-prevalence and associations with other parasites. Ann Trop Med Parasitol 86:129–136

    CAS  PubMed  Google Scholar 

  • Böhm-Gloning B, Knobloch J, Walderich B (1997) Five subgroups of Blastocystis hominis isolates from symptomatic and asymptomatic patients revealed by restriction site analysis of PCR-amplified 16S-like rDNA. Trop Med Int Health 2:771–778

    Article  PubMed  Google Scholar 

  • Clark CG (1997) Extensive genetic diversity in Blastocystis hominis. Mol Biochem Parasitol 87:79–83

    Article  CAS  PubMed  Google Scholar 

  • Garcia SL, Bruckner DA (1997) Macroscopic and microscopic examination of fecal specimens. In: Garcia SL, Bruckner DA (eds) Diagnostic medical parasitology, 3rd edn. American Society for Microbiology, Washington, DC., pp 608–651,

  • Hussain Qadri SM, Al-Okaili GA, Al-Dayel F (1989) Clinical significance of Blastocystis hominis. J Clin Microbiol 27:2407–2409

    PubMed  Google Scholar 

  • Jones WR (1946) The experimental infection of rats with Entamoeba histolytica. Ann Trop Med Parasitol 40:130–140

    Google Scholar 

  • Leelayoova S, Taamasri P, Rangsin R, Naaglor T, Thathaisong U, Mungthin M (2002) In vitro cultivation: a sensitive method for detecting Blastocystis hominis. Ann Trop Med Parasitol 96:803–807

    Article  CAS  PubMed  Google Scholar 

  • Nimri LF (1993) Evidence of an epidemic of Blastocystis hominis infections in preschool children in northern Jordan. J Clin Microbiol 31:2706–2708

    CAS  PubMed  Google Scholar 

  • Noël C, Peyronnet C, Gerbod D, Edgcomb VP, Delgado-Viscogliosi P, Sogin ML, Capron M, Viscogliosi E, Zenner L (2003) Phylogenetic analysis of Blastocystis isolates from different hosts based on the comparison of small-subunit rRNA gene sequences. Mol Biochem Parasitol 126:119–123

    Article  PubMed  Google Scholar 

  • Stenzel DJ, Boreham PFL (1996) Blastocystis hominis revisited. Clin Microbiol Rev 9:563–584

    CAS  PubMed  Google Scholar 

  • Taamasri P, Leelayoova S, Rangsin R, Naaglor T, Ketupanya A, Mungthin M (2002) Prevalence of Blastocystis hominis carriage in Thai army personnel based in Chonburi, Thailand. Mil Med 167:643–646

    PubMed  Google Scholar 

  • Thathaisong U, Worapong J, Mungthin M, Tan-Ariya P, Viputtigul K, Sudatis A, Noonai A, Leelayoova S (2003) Blastocystis isolates from a pig and a horse are closely related to Blastocystis hominis. J Clin Microbiol 41:967–975

    Article  PubMed  Google Scholar 

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Acknowledgements

This work was financially supported by the Thailand Tropical Diseases Research Programme (T2) (ID 00-1-HEL-24-011). The experiments comply with the current laws of Thailand.

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Authors and Affiliations

  1. Department of Pathology, Phramongkutklao College of Medicine, 315 Ratchawithi Road, Ratchathewi, 10400, Bangkok , Thailand

    Sumeth Termmathurapoj

  2. Department of Parasitology, Phramongkutklao College of Medicine, 315 Ratchawithi Road, Ratchathewi, 10400, Bangkok , Thailand

    Saovanee Leelayoova, Thirayost Nimmanon, Paanjit Taamasri & Mathirut Mungthin

  3. Department of Military and Community Medicine, Phramongkutklao College of Medicine, 315 Ratchawithi Road, Ratchathewi, 10400, Bangkok , Thailand

    Pote Aimpun

  4. Burapha University, 20131, Chonburi , Thailand

    Umaporn Thathaisong

Authors
  1. Sumeth Termmathurapoj
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  2. Saovanee Leelayoova
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  3. Pote Aimpun
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  4. Umaporn Thathaisong
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  5. Thirayost Nimmanon
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  6. Paanjit Taamasri
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  7. Mathirut Mungthin
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Correspondence to Mathirut Mungthin.

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Termmathurapoj, S., Leelayoova, S., Aimpun, P. et al. The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens. Parasitol Res 93, 445–447 (2004). https://doi.org/10.1007/s00436-004-1157-x

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  • DOI: https://doi.org/10.1007/s00436-004-1157-x

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