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A three-platelet mRNA set: MAX, MTURN and HLA-B as biomarker for lung cancer

  • Original Article – Cancer Research
  • Published:
Journal of Cancer Research and Clinical Oncology Aims and scope Submit manuscript

Abstract

Background

During the development of tumors, tumors “educate” platelets causing changes in their mRNAs expression profiles and phenotypes, thereby, tumor-educated platelet (TEP) mRNA profile has the potential to diagnose lung cancer. The current study aimed to examine whether TEPs might be a potential biomarker for lung cancer diagnostics.

Methods

Platelet precipitation was obtained by low-speed centrifugation and subjected to Trizol for total RNA extraction. Platelet MAX, MTURN, and HLA-B mRNA were selected by microarray, validated by qPCR, and analyzed combined with related clinical factors.

Results

Our results showed that a three-platelet mRNA set: MAX, MTURN, and HLA-B was significantly up-regulated in lung cancer patients as well as in early-stage lung cancer patients compared with those from healthy donors, the area under the curve (AUC) was 0.734, 0.787, respectively, among which platelet MTURN mRNA processed a dramatically high diagnostic efficiency in female patients with lung cancer, its AUC for female was 0.825. More importantly, the three-platelet mRNA set: MAX, MTURN, and HLA-B was associated with chemotherapeutic effect, low mRNA expression of this three-platelet set was correlated with “favorable” first chemotherapy response.

Conclusions

A three-platelet mRNA set: MAX, MTURN and HLA-B enables blood-based lung cancer diagnosis and chemotherapy response prediction.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (81773237, 81672104), the Shandong Provincial Key Research and Development Program (2016GSF201211, 2016GSF201162, 2017GSF18183 and 2017CXGC1207).

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Author notes

  1. Lele Liu and Xingguo Song contributed equally to this work.

Authors and Affiliations

  1. School of Medicine and Life Sciences, University of Jinan, Shandong Academy of Medical Sciences, Jinan, China

    Lele Liu, Xinyi Li & Limin Niu

  2. Department of Clinical Laboratory, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China

    Lele Liu, Xinyi Li, Linlin Xue, Shanshan Ding, Limin Niu, Li Xie & Xianrang Song

  3. Shandong Provincial Key Laboratory of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China

    Xingguo Song

  4. Department of Clinical Laboratory, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, China

    Shanshan Ding

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  1. Lele Liu
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  2. Xingguo Song
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Correspondence to Xianrang Song.

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432_2019_3032_MOESM1_ESM.tif

Supplementary material 1 Platelet quality control. Platelet purity was detected by wright-giemsa dye under a microscope (a), white arrow indicated the red blood cells, and activation state (b) of the platelet was detected by flow cytometry (TIFF 25418 kb)

432_2019_3032_MOESM2_ESM.tif

Supplementary material 2 Platelet UQCRH mRNA expression as biomarker for lung cancer. a Platelet UQCRH mRNA was elevated in lung cancer significantly. LC, patients with lung cancer; HD, healthy donors. b Low mRNA expression of UQCRH was correlated with favorable first chemotherapy response. PR, partial response. *P < 0.05. (TIFF 5418 kb)

Supplementary material 3 (DOCX 31 kb)

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Liu, L., Song, X., Li, X. et al. A three-platelet mRNA set: MAX, MTURN and HLA-B as biomarker for lung cancer. J Cancer Res Clin Oncol 145, 2713–2723 (2019). https://doi.org/10.1007/s00432-019-03032-9

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  • DOI: https://doi.org/10.1007/s00432-019-03032-9

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