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miR-708 promotes the development of bladder carcinoma via direct repression of Caspase-2

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Abstract

Purpose

Bladder cancer is one of the world’s top ten malignant tumors. The crucial role of microRNA in carcinogenesis has been well emphasized. Considering miRNA expression was tumor stage-, tissue-, or even development-specific, more experimental evidences about the functions of miRNAs in bladder cancer should be discovered to advance applying of miRNA in the diagnosis or therapy of cancer.

Methods

MiR-708 level in bladder carcinoma and adjacent noncancerous tissues was tested by real-time qPCR. Cell apoptosis was analyzed by using flow cytometry. The tumorigenicity of bladder carcinoma cells was evaluated in nude mice model. Luciferase reporter gene assays were performed to identify the interaction between miR-708 and 3′UTR of Caspase-2 mRNA. The protein level of Caspase-2 was determined by western blotting.

Results

In this study, we reported that miR-708 was frequently dysregulated in human bladder carcinoma tissues compared to normal tissues. In addition, we found that silencing of miR-708 could promote the T24 and 5637 cells to apoptosis and inhibit the bladder tumor growth in vivo. Also, Caspase-2 was proved to be one of direct targets of miR-708 in T24 and 5637 cells. Further results showed that Caspase-2 was involved in the miR-708 regulated cell apoptosis.

Conclusions

All together, these results suggest miR-708 may act as an oncogene and induce the carcinogenicity of bladder cancer by down-regulating Caspase-2 level.

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Conflict of interest

The authors declare that they have no conflict of interest. We have no financial and personal relationships with other people or organizations that can inappropriately influence our work. There is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the position presented in the manuscript.

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Authors and Affiliations

  1. Department of Urology, Clinical Division of Surgery, Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing, 100853, China

    Tao Song, Xu Zhang, Lei Zhang, Jun Dong, Wei Cai, Jiangping Gao & Baofa Hong

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  1. Tao Song
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  2. Xu Zhang
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  3. Lei Zhang
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  4. Jun Dong
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  5. Wei Cai
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  7. Baofa Hong
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Corresponding author

Correspondence to Tao Song.

Electronic supplementary material

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432_2013_1392_MOESM1_ESM.tif

Analysis of miR-708 expression in cancer cell lines and human bladder cancer tissue by northern blot. 1, adjacent noncancerous tissue. 2, bladder cancer tissue. 3, 5637 cells. 4, HepG2 cells. 5, Hela cells. 6,T24 cells. (TIFF 813 kb)

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Song, T., Zhang, X., Zhang, L. et al. miR-708 promotes the development of bladder carcinoma via direct repression of Caspase-2. J Cancer Res Clin Oncol 139, 1189–1198 (2013). https://doi.org/10.1007/s00432-013-1392-6

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  • DOI: https://doi.org/10.1007/s00432-013-1392-6

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