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BRG1 expression is increased in human glioma and controls glioma cell proliferation, migration and invasion in vitro

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Abstract

Purpose

The purposes of our study were to elucidate the role of BRG1 in the development of human glioma and to determine the effect of BRG1 on glioma cell growth, migration and invasion.

Methods

Using tissue microarray and immunohistochemistry, we evaluated BRG1 staining in 190 glioma tissues, 8 normal brain tissues and 8 tumor adjacent normal brain tissues. We studied glioma cell proliferative ability with reduced BRG1 expression by siRNA using CCK-8 cell proliferation assay and cell cycle analysis. We studied the role of BRG1 in glioma cell migration and invasion by cell migration assay and matrigel invasion assay. We performed western blot to detect cyclin D1, cyclin B1 and MMP-2 protein expression. We also detected MMP-2 enzyme activity by gelatin zymography.

Results

Our results showed that BRG1 expression was increased in benign tumor and malignant tumor compared with tumor adjacent normal brain tissue (P < 0.01 for both). We did not find any correlation between BRG1 expression and clinicopathological parameters. In addition, we found that knockdown of BRG1 in glioma cell lines inhibits cell growth due to the G1 phase arrest by downregulating cyclin D1. We further demonstrated that silencing of BRG1 in glioma cells inhibited the cell migration and invasion abilities, and downregulation of MMP-2 expression greatly contributed to the reduced cell invasion and migration abilities.

Conclusions

Our data indicated that BRG1 expression is significantly increased in human glioma and it may be involved in the process of glioma cell proliferation, migration and invasion.

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Acknowledgments

This project is supported by grants from the National Natural Science Foundation of China (No. 30972976, 81071854), the Science and Technology Department of Jiangsu province (No. BK2009089) and Xuzhou Medical College (No. 2010KJZ03, 2010KJZ04).

Conflict of interest

We declare that we have no conflict of interest.

Author information

Authors and Affiliations

  1. Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou, 221002, China

    Jin Bai & Jun-Nian Zheng

  2. Department of Neurosurgery, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, Jiangsu Province, China

    Peng-Jin Mei & Zheng-Quan Yu

  3. School of Pathology, Xuzhou Medical College, Xuzhou, 221002, China

    Hui Liu & Yong-Ping Wu

  4. The First Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221002, China

    Hui Liu, Chen Li, Wang Li & Jun-Nian Zheng

  5. Laboratory of Biological Cancer Therapy, Xuzhou Medical College, 84 West Huaihai Road, Xuzhou, 221002, Jiangsu Province, China

    Jun-Nian Zheng

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  1. Jin Bai
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Corresponding authors

Correspondence to Zheng-Quan Yu or Jun-Nian Zheng.

Additional information

Jin Bai and Peng-Jin Mei contributed equally to this paper.

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Bai, J., Mei, PJ., Liu, H. et al. BRG1 expression is increased in human glioma and controls glioma cell proliferation, migration and invasion in vitro. J Cancer Res Clin Oncol 138, 991–998 (2012). https://doi.org/10.1007/s00432-012-1172-8

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  • DOI: https://doi.org/10.1007/s00432-012-1172-8

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