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Cytoarchitecture of the mouse neocortex revealed by the low-molecular-weight neurofilament protein subunit

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Abstract

The expression patterns of the medium- and high-molecular-weight subunits of the neurofilament protein triplet have been extensively studied in several neuroanatomical studies. In the present study, we report the use of the low-molecular-weight neurofilament protein subunit (NF-L) as a reliable marker within the neurofilament protein family to reveal the regional architecture of mammalian neocortex. We document clearly its usefulness in anatomical parcellation studies and report unique expression patterns of NF-L throughout the mouse neocortex. NF-L was most abundant in the somatosensory cortex, the lateral secondary visual area, the granular insular cortex, and the motor cortex. Low NF-L staining intensity was observed in the agranular insular cortex, the prelimbic and infralimbic cortex, the anterior cingulate cortex, the visual rostromedial areas, the temporal association cortex, the ectorhinal cortex, and the lateral entorhinal cortex. NF-L immunoreactivity was present in the perikarya, dendrites, and proximal segment of axons primarily of pyramidal neurons, and was mainly located in layers II and III, and to a lesser extent in layers V and VI. Interestingly, Black-Gold myelin staining confirmed a close correlation between NF-L immunoreactivity and myelination patterns. The characteristic and distinctive distribution and laminar expression profiles of NF-L make it an excellent tool to assess accurately topographical boundaries among neocortical areas as illustrated herein in the adult mouse brain.

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Abbreviations

ACd:

Dorsal anterior cingulate cortex

ACv:

Ventral anterior cingulate cortex

AC/RS:

Anterior cingulate/retrosplenial cortex

ac:

Anterior commissure

AI:

Agranular insular cortex

AId:

Dorsal agranular insular cortex

AIv:

Ventral agranular insular cortex

AIp:

Posterior agranular insular cortex

Au1:

Primary auditory cortex

Aud:

Dorsal auditory cortex

Auv:

Ventral auditory cortex

DI:

Dysgranular insular cortex

DLO:

Dorsolateral orbital cortex

Ect:

Ectorhinal cortex

ec:

External capsule

GI:

Granular insular cortex

IL:

Infralimbic cortex

LEnt:

Lateral entorhinal cortex

LO:

Lateral orbital cortex

lo:

Lateral olfactory tract

M1:

Primary motor cortex

M2:

Secondary motor cortex

MEnt:

Medial entorhinal cortex

MO:

Medial orbital cortex

NF-L:

Neurofilament protein low molecular weight subunnit

Pir:

Piriform cortex

Prh:

Perirhinal cortex

PL:

Prelimbic cortex

RM1:

Rostromedial visual area 1

RM2:

Rostromedial visual area 2

RM3–4:

Rostromedial visual area 3–4

RSA:

Agranular retrosplenial cortex

RSG:

Granular retrosplenial cortex

S1:

Primary somatosensory cortex

S1BF:

Barrel field in S1

S2:

Secondary somatosensory cortex

TeA:

Temporal association area

V1:

Primary visual cortex

V2L:

Lateral secondary visual cortex

V2M:

Medial secondary visual cortex

VO:

Ventral orbital cortex

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Acknowledgments

We thank Inge Van der Auwera, Lieve Geenen and Ria Vanlaer for excellent technical assistance. This study was supported by the Fund for Scientific Research—Flanders, Belgium (FWO-Flanders and the Research Fund of the Katholieke Universiteit Leuven, Belgium, grant OT 09/22 (to L.A.), and by the James S. McDonnell Foundation, grant 22002078 (to P.R.H.).

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  1. Laboratory of Neuroplasticity and Neuroproteomics, Katholieke Universiteit Leuven, Naamsestraat 59, 3000, Leuven, Belgium

    Melissa Paulussen, Sandy Jacobs, Estelle Van der Gucht & Lutgarde Arckens

  2. Department of Neuroscience, Mount Sinai School of Medicine, New York, NY, 10029, USA

    Patrick R. Hof

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  1. Melissa Paulussen
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  2. Sandy Jacobs
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  3. Estelle Van der Gucht
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  4. Patrick R. Hof
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  5. Lutgarde Arckens
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Correspondence to Lutgarde Arckens.

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Paulussen, M., Jacobs, S., Van der Gucht, E. et al. Cytoarchitecture of the mouse neocortex revealed by the low-molecular-weight neurofilament protein subunit. Brain Struct Funct 216, 183–199 (2011). https://doi.org/10.1007/s00429-011-0311-3

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