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Comparison of cyclooxygenase-2 and CD44 mRNA expression in colorectal cancer and its relevance for prognosis

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Abstract

This study evaluated CD44 and COX-2 expression in colorectal cancer (CRC) and analyzed its relationship with the clinicopathological characteristics. The prognostic impact on patient survival was compared between the two proteins. CD44 and COX-2 mRNA levels in 42 primary CRCs were analyzed using quantitative real-time PCR, with normalization relative to GAPDH. The cycle threshold (Ct) values were measured, and results are expressed as the Ct ratios of CD44 or COX-2 to GAPDH. The COX-2 Ct ratio was much lower in cases of lymphovascular invasion by the tumor than for no invasion (P = 0.004). During follow-up for a median of 40 months, there was no significant difference in the median CD44 Ct ratio between survivors and non-survivors (P = 0.362), whereas the COX-2 Ct ratio was significantly associated with survival at the time of data analysis (P = 0.042). The survival of colorectal cancer patients with a high COX-2 Ct ratio was significantly longer than that of patients with a low COX-2 Ct ratio (P = 0.048). This study suggests that COX-2 expression has a more significant impact than CD44 expression on the survival of CRC patients. Further studies are needed to resolve these issues with a large sample size.

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Acknowledgment

This work was supported by a Research Grant (0720570) from the National Cancer Center, South Korea.

Conflict of interest statement

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Surgery, Chonnam National University Hwasun Hospital and Medical School, 160 Ilsimri, Hwasun-eup, Hwasun-gun, Gwangju, 519-809, Jeonnam, South Korea

    Jung Wook Huh, Hyeong Rok Kim & Young Jin Kim

  2. Department of Pathology, Chonnam National University Hwasun Hospital and Medical School, Gwangju, South Korea

    Jae Hyuk Lee

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  1. Jung Wook Huh
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  2. Hyeong Rok Kim
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  3. Jae Hyuk Lee
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  4. Young Jin Kim
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Correspondence to Hyeong Rok Kim.

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Huh, J.W., Kim, H.R., Lee, J.H. et al. Comparison of cyclooxygenase-2 and CD44 mRNA expression in colorectal cancer and its relevance for prognosis. Virchows Arch 454, 381–387 (2009). https://doi.org/10.1007/s00428-009-0752-8

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  • DOI: https://doi.org/10.1007/s00428-009-0752-8

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