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The detection of CD14 and CD16 in paraffin-embedded bone marrow biopsies is useful for the diagnosis of chronic myelomonocytic leukemia

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Abstract

Histopathological study of bone marrow biopsy (BMB) in chronic myelomonocytic leukemia (CMML) is often difficult and might benefit from an immunohistochemical approach. We immunostained 15 cases of CMML, focusing at two new antibodies staining for CD14 and CD16 on paraffin-embedded tissues. CD68 (KP1), CD68 (PG-M1), and CD163 were not differentially expressed between CMML and chronic myelogenous leukemia (CML). In CMML BMB, we found a significant increase in the number of CD14+ monocytes. This increase was made of dispersed cells in the interstitium, often exhibiting bilobated nuclei, and being difficult to differentiate from neutrophils. There was no expansion of CD16+ monocyte-like cells. However, we found a significant decrease in the number of granulocytes expressing CD16, MPO, and CD15 in CMML compared to CML and control BMB, probably related to dysgranulopoiesis. Indeed, BMB immunohistochemistry can be helpful in CMML by identifying both the monocyte expansion with CD14 and the dysgranulopoiesis with CD16.

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Acknowledgments

The authors wish to thank Patricia Bonjour and Pierre-Etienne Bouchet for technical help. Marwan Qubaja is supported by a grant in cooperation between University Pierre et Marie Curie and Al-Quds University—School of Medicine.

Conflict of interest

The authors do not identify any conflict of interest to declare.

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Authors and Affiliations

  1. Service d’Anatomie et de Cytologie Pathologiques “Jacques Delarue”, AP-HP, Hôtel Dieu, 1, Place du Parvis Notre-Dame, 75181, Paris Cedex 04, France

    Marwan Qubaja, Béatrice Marmey, Agnès Le Tourneau, Jacques Diebold, Josée Audouin & Thierry Jo Molina

  2. Université Pierre et Marie Curie, Paris, France

    Marwan Qubaja, Bettina Fabiani & Jean-Pierre Marie

  3. Faculté de Médecine, Université Paris Descartes, Paris, France

    Agnès Le Tourneau, Jacques Diebold, Josée Audouin, Frédéric Geissmann & Thierry Jo Molina

  4. Service d’Hématologie clinique, Hôtel Dieu, Paris, France

    Stéphanie Haiat & Jean-Pierre Marie

  5. INSERM, U838, Institut Necker-Enfants Malades, Paris, France

    Frédéric Geissmann & Thierry Jo Molina

  6. Service d’Anatomie Pathologique, Université Paris Diderot; AP-HP, Hôpital Beaujon, Paris, France

    Dominique Cazals-Hatem

  7. Service d’Anatomie Pathologique, AP-HP, Hôpital Saint-Antoine, Paris, France

    Bettina Fabiani

Authors
  1. Marwan Qubaja
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  2. Béatrice Marmey
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  3. Agnès Le Tourneau
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  4. Stéphanie Haiat
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  5. Dominique Cazals-Hatem
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  6. Bettina Fabiani
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  7. Jacques Diebold
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  8. Jean-Pierre Marie
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  9. Josée Audouin
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  10. Frédéric Geissmann
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  11. Thierry Jo Molina
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Corresponding author

Correspondence to Thierry Jo Molina.

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Qubaja, M., Marmey, B., Le Tourneau, A. et al. The detection of CD14 and CD16 in paraffin-embedded bone marrow biopsies is useful for the diagnosis of chronic myelomonocytic leukemia. Virchows Arch 454, 411–419 (2009). https://doi.org/10.1007/s00428-009-0726-x

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  • DOI: https://doi.org/10.1007/s00428-009-0726-x

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