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Establishment of novel human dedifferentiated chondrosarcoma cell line with osteoblastic differentiation

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Abstract

Dedifferentiated chondrosarcoma is a rare, highly malignant variant of chondrosarcoma in which a high-grade sarcoma coexists with a low-grade chondroid tumor. We herein review a case of dedifferentiated chondrosarcoma with an osteosarcoma omit component that occurred in the distal femur of a 38-year-old man. We established the cell line (NDCS-1) from a pleural effusion of the metastatic lung tumor. The cell line was characterized by a the G-banded karyotype, polymerase chain reaction (PCR) single-strand conformation polymorphism analysis, spectral karyotyping, and reverse transcriptase PCR (RT-PCR). The tumor exhibited complex karyotypes and a high frequency of chromosomal amplication with p53 mutation. This tumor revealed an osteoblastic and chondroblastic character in vitro and in severe combined immunodeficiency mice. The expression and phosphorylation of platelet-derived growth factor receptor-β, which seemed to play a major role in the malignant phenotype of chondrosarcoma, was confirmed by RT-PCR and Western blotting. To our knowledge, this is the first report of the establishment of a human dedifferentiated chondrosarcoma.

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Acknowledgments

The authors thank S. Urakawa, H. Ishigaki, M. Sugai, H. Akazawa, and Y. Tanaka for their technical assistance.

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Authors and Affiliations

  1. Division of Orthopaedic Surgery,Department of Regenerative Transplant Medicine, Niigata University Graduate School of Medical and Dental Sciences, 757-1 Asahimachi-dori Ichiban-cho, Niigata City, Niigata, 951-8510, Japan

    Naoko Kudo, Akira Ogose, Tetsuo Hotta, Hiroyuki Kawashima, Wenguang Gu & Naoto Endo

  2. Section of Pathology, Niigata University Medical and Dental Hospital, 757 Asahimachi-dori Ichiban-cho, Niigata City, Niigata, 951-8510, Japan

    Hajime Umezu

  3. Center for Molecular Biology and Cytogenetics, SRL, Tokyo, Japan

    Tsuyoshi Toyama

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  1. Naoko Kudo
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  2. Akira Ogose
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  3. Tetsuo Hotta
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  4. Hiroyuki Kawashima
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  7. Tsuyoshi Toyama
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Correspondence to Akira Ogose.

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Kudo, N., Ogose, A., Hotta, T. et al. Establishment of novel human dedifferentiated chondrosarcoma cell line with osteoblastic differentiation. Virchows Arch 451, 691–699 (2007). https://doi.org/10.1007/s00428-007-0426-3

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