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New cell lines with chondrocytic phenotypes from human chondrosarcoma

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Abstract

In the present study, we investigated chondrocytic characterization for newly established human chondrosarcoma cell lines. A chondrosarcoma cell line, HCS-TG, was established by the implantation of grade-2 human chondrosarcoma into athymic mice. Cloning of HCS-TG cells from passage 17 was performed. After cell cloning, two clonal-cell lines (HCS-TG C3 and E2) with good proliferative activities were obtained. These cell lines in monolayer culture retained a polygonal morphology. Their doubling times were 68 h and 45 h, respectively. mRNA expressions of type-I, -II, -X, and -XI collagens and aggrecan core protein were detected on reverse-transcription polymerase chain reaction. Protein expression of type-II collagen was confirmed in each cell line using Western blotting. However, there was no expression of type-I collagen. Moreover, gelatin zymography revealed that both cell lines produced extracellular matrices with matrix metalloproteinases 2 and 9. The parental HCS-TG cells had tumorigenicity in athymic mice; however, C3 and E2 were not tumorigenic. New clonal-cell lines HCS-TG C3 and E2 derived from human chondrosarcoma are morphologically chondrocytic in serial monolayer cultures and express chondrocytic phenotypes.

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Acknowledgements

We are grateful to Noriyuki Tsumaki, M.D. Ph.D., Takanobu Nakase, M.D. Ph.D., and Tetsuya Tomita, M.D. Ph.D., for provision of primers of collagens and to Akihiko Matsumine, M.D. Ph.D., and Yukihiro Isogai, Ph.D., for technical assistance.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Osaka National Hospital, Osaka 2-1-14 Hoenzaka, Chuo-ku, 540-0006, Osaka , Japan

    Ikuo Kudawara

  2. Department of Orthopaedic Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka 1-3-3 Nakamichi, Higashinari-ku, 537-8511, Osaka , Japan

    Nobuhito Araki

  3. Department of Orthopaedics, Osaka University Graduate School of Medicine, Suita 2-2 Yamadaoka, 565-0871, Suita , Japan

    Akira Myoui & Hideki Yoshikawa

  4. Clinical Development Department Asahi Kasei Pharma Corporation, 9-1 Kanda Mitoyo-cho, Chiyoda-ku, 101-8481, Tokyo , Japan

    Yoichi Kato

  5. Department of Orthopaedic Surgery, Faculty of Medicine, Mie University, Tsu, 2-74 Edobashi, 514-0001, Tsu , Japan

    Atsumasa Uchida

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  1. Ikuo Kudawara
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  2. Nobuhito Araki
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  3. Akira Myoui
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Correspondence to Ikuo Kudawara.

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No benefits in any form have been received or will be received from a commercial party related directly or indirectly to the subject of this article. This work was partly supported by the grants from the Japanese Ministry of Education, Science and Culture.

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Kudawara, I., Araki, N., Myoui, A. et al. New cell lines with chondrocytic phenotypes from human chondrosarcoma. Virchows Arch 444, 577–586 (2004). https://doi.org/10.1007/s00428-004-1020-6

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  • DOI: https://doi.org/10.1007/s00428-004-1020-6

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