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TGFβ signaling in Tribolium: vertebrate-like components in a beetle

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Abstract

The cytokines of the TGFβ superfamily are highly conserved in evolution and elicit a diverse range of cellular responses in all metazoa. In Drosophila, the signaling pathways of the two TGFβ subfamilies, Activins and Bone Morphogenetic Proteins (BMPs), have been well studied. To address the question of whether the findings from Drosophila are representative of insects in general, we analyzed the components of TGFβ-signaling present in the genome of the beetle Tribolium castaneum. We were able to identify orthologs of the BMPs Decapentaplegic and Glass bottom boat, of the Activins Activinβ and Dawdle, as well as orthologs of the less well-known ligands Myoglianin and Maverick, together with orthologs of all TGFβ receptors and cytoplasmic signal transducers present in Drosophila. This indicates that the diversity of TGFβ signaling components is generally well conserved between Drosophila and Tribolium. However, the genome of the beetle—and of the bee Apis mellifera—lacks an ortholog of the Drosophila BMP Screw but does contain a vertebrate-like BMP10 homolog which is not found in Drosophila. Concerning BMP inhibitors, Tribolium displays an even more vertebrate-like ensemble of components. We found two orthologs of the vertebrate DAN family, Dan and Gremlin, and show embryonic expression of a vertebrate-like BAMBI ortholog, all of which are absent in Drosophila. This suggests that Tribolium might have retained a more ancestral composition of TGFβ signaling components and that TGFβ signaling underwent considerable change in the Drosophila lineage. Tribolium is an excellent model to study the function of these ancestral signaling components in insects.

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Acknowledgments

We thank Eva Nowack and Birger Marin for help with the phylogenetic analyses. Alfonso Martinez-Arias provided an aliquot of the pMAD antibody. Alistair McGregor improved the English. M.v.d.Z. was financed by the SFB 680, R.N.d.F by the International Graduate School in Genetics and Functional Genomics of Cologne.

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  1. Institute for Genetics, University of Cologne, Zülpicher Straße 47, 50674, Cologne, Germany

    Maurijn Van der Zee

  2. Institute for Developmental Biology, University of Cologne, Gyrhofstraße 17, 50931, Cologne, Germany

    Rodrigo Nunes da Fonseca & Siegfried Roth

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  1. Maurijn Van der Zee
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  2. Rodrigo Nunes da Fonseca
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  3. Siegfried Roth
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Correspondence to Maurijn Van der Zee.

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Van der Zee, M., da Fonseca, R. & Roth, S. TGFβ signaling in Tribolium: vertebrate-like components in a beetle. Dev Genes Evol 218, 203–213 (2008). https://doi.org/10.1007/s00427-007-0179-7

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