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TabZIP60 is involved in the regulation of ABA synthesis-mediated salt tolerance through interacting with TaCDPK30 in wheat (Triticum aestivum L.)

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Abstract

Main conclusion

TabZIP60 is found to interact with TaCDPK30 and act as a positive regulator of ABA synthesis-mediated salt tolerance in wheat.

Abstract

Wheat basic leucine zipper (bZIP) transcription factor (TabZIP60) was previously found to act as a positive regulator of salt resistance. However, its molecular mechanism in response to salt stress in wheat is still unclear. In this study, TabZIP60 was found to interact with wheat calcium-dependent protein kinase (TaCDPK30), which belonged to group III of CDPK family, and was induced by salt, polyethylene glycol, and abscisic acid (ABA) treatments. This mutation of serine 110 in TabZIP60 resulted in no interaction with TaCDPK30. Moreover, TaCDPK30 was involved in interactions with wheat protein phosphatase 2C clade A (TaPP2CA116/TaPP2CA121). TabZIP60-overexpressing wheat plants showed increased salt tolerance, as exhibited by better growth status, higher soluble sugar, and lower malonaldehyde contents of transgenic plants than wild-type wheat cv. Kenong 199 under salt stress. Moreover, transgenic lines showed high ABA content by upregulating ABA synthesis-related gene expression levels. TabZIP60 protein could bind and interact with the promoter of the wheat nine-cis epoxycarotenoid dioxygenase (TaNCED2) gene. Furthermore, TabZIP60 upregulated several stress response gene expression levels, which could also increase the plant’s ability to resist salt stress. Thus, these results suggest that TabZIP60 could function as a regulator of ABA synthesis-mediated salt tolerance through interacting with TaCDPK30 in wheat.

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Abbreviations

bZIP:

Basic leucine zipper

CDPK:

Calcium-dependent protein kinase

ABA:

Abscisic acid

PP2CA:

Protein phosphatase 2C clade A

ABF:

ABA-responsive element-binding factor

AREB:

ABA-responsive element-binding protein

NCED:

Nine-cis epoxycarotenoid dioxygenase

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Acknowledgements

This study was supported by the National Natural Science Foundation of China [31660392, 32260471], the Academic Backbone Project from the Northwest Normal University [2019GG-3], and the Young Teachers Improving Program from the Northwest Normal University [NWNU-LKQN-14-11].

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Authors and Affiliations

  1. College of Life Sciences, Northwest Normal University, Lanzhou, 730070, Gansu, China

    Lina Zhang, Lijuan Zhao, Liting Wang, Xingyan Liu, Zhen Yu, Jing Liu, Wangze Wu & Lan Ding

  2. Key Laboratory for Crop Gene Resources and Germplasm Enhancement, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, MOA, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Chuan Xia, Lichao Zhang & Xiuying Kong

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  1. Lina Zhang
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Correspondence to Lina Zhang.

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Zhang, L., Zhao, L., Wang, L. et al. TabZIP60 is involved in the regulation of ABA synthesis-mediated salt tolerance through interacting with TaCDPK30 in wheat (Triticum aestivum L.). Planta 257, 107 (2023). https://doi.org/10.1007/s00425-023-04141-z

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