Abstract
Main conclusion
We identified IAA13 negatively associated with lateral root number by comparing the differential expressed genes between Bupleurum chinense and B. scorzonerifolium.
Abstract
Dried roots of the genus Bupleurum L. are used as a herbal medicine for diseases in Asia. Bupleurum chinense has a greater number of lateral roots than B. scorzonerifolium, but the genetic mechanisms for such differences are largely unknown. We (a) compared the transcriptome profiles of the two species and (b) identified a subset of candidate genes involved in auxin signal transduction and explored their functions in lateral root development. By isoform sequencing (Iso-Seq) analyses of the whole plant, more unigenes were found in B. scorzonerifolium (118,868) than in B. chinense (93,485). Given the overarching role of indole-3-acetic acid (IAA) as one of the major regulators of lateral root development, we identified 539 unigenes associated with auxin signal transduction. Fourteen and 44 unigenes in the pathway were differentially expressed in B. chinense and B. scorzonerifolium, respectively, and 3 unigenes (LAX2, LAX4, and IAA13) were expressed in both species. The number of lateral root primordia increased after exogenous auxin application at 8 h and 12 h in B. scorzonerifolium and B. chinense, respectively. Since overexpression of IAA13 in Arabidopsis reduced the number of lateral roots, we hypothesized that IAA13 is involved in the reduction of the number of lateral roots in B. scorzonerifolium.
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Abbreviations
- ARF:
-
Auxin response factor
- DEG:
-
Differentially expressed genes
- Iso-Seq:
-
Isoform sequencing
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Acknowledgements
The authors would like to acknowledge and thank Dr. Victor Resco de Dios from Joint Research Unit CTFC–AGROTECNIO and Dr. Jing Wu from the Chinese Academy of Agricultural Sciences for paper editing.
Funding
This work was supported by grants to the Southwest University of Science and Technology Chinese Medical Plant Breeding Program from the National Natural Science Foundation of China (81603223), Agriculture Research System of China under grant number CARS-21, CAMS Innovation Fund for Medical Sciences (CIFMS) under grant number 2016-I2M-2-003, National Transgenic Major Project of China (2019ZX08010004-005), Crop and Livestock Breeding Project in Sichuan (2021YFYZ0012-13), and Programs of Science and Technology Department of Sichuan Province (2019YFH0072 and 2019YJ0443) and doctoral research funding from the Southwest University of Science and Technology (19zx7117).
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Yu, M., Chen, H., Liu, Q. et al. Analysis of unigenes involved in lateral root development in Bupleurum chinense and B. scorzonerifolium. Planta 253, 128 (2021). https://doi.org/10.1007/s00425-021-03644-x
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DOI: https://doi.org/10.1007/s00425-021-03644-x