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Transcriptome analysis of root-knot nematode (Meloidogyne incognita)-resistant and susceptible sweetpotato cultivars

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Abstract

Main conclusion

Transcriptome analysis was performed on the roots of susceptible and resistant sweetpotato cultivars infected with the major root-knot nematode species Meloidogyne incognita. In addition, we identified a transcription factor-mediated defense signaling pathway that might function in sweetpotato–nematode interactions.

Root-knot nematodes (RKNs, Meloidogyne spp.) are important sedentary endoparasites of many agricultural crop plants that significantly reduce production in field-grown sweetpotato. To date, no studies involving gene expression profiling in sweetpotato during RKN infection have been reported. Therefore, in the present study, transcriptome analysis was performed on the roots of susceptible (cv. Yulmi) and resistant (cv. Juhwangmi) sweetpotato cultivars infected with the widespread, major RKN species Meloidogyne incognita. Using the Illumina HiSeq 2000 platform, we generated 455,295,628 pair-end reads from the fibrous roots of both cultivars, which were assembled into 74,733 transcripts. A number of common and unique genes were differentially expressed in susceptible vs. resistant cultivars as a result of RKN infection. We assigned the differentially expressed genes into gene ontology categories and used MapMan annotation to predict their functional roles and associated biological processes. The candidate genes including hormonal signaling-related transcription factors and pathogenesis-related genes that could contribute to protection against RKN infection in sweetpotato roots were identified and sweetpotato–nematode interactions involved in resistance are discussed.

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Abbreviations

DEG:

Differentially expressed genes

ET:

Ethylene

JA:

Jasmonic acid

PR:

Pathogenesis-related

RKN:

Root-knot nematode

SA:

Salicylic acid

TF:

Transcription factor

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Acknowledgements

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, and Future Planning (2018R1A1A1A05018446).

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Author notes

  1. Il Hwan Lee, Donghwan Shim, and Jea Cheol Jeong contributed equally to this work.

Authors and Affiliations

  1. Department of Forest Genetic Resources, National Institute of Forest Science, Suwon, 16631, Republic of Korea

    Il Hwan Lee & Donghwan Shim

  2. Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Republic of Korea

    Jea Cheol Jeong

  3. Department of Biology Education, IALS, Gyeongsang National University, Jinju, 660-701, Republic of Korea

    Yeon Woo Sung, Ki Jung Nam & Yun-Hee Kim

  4. Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan, Republic of Korea

    Jung-Wook Yang

  5. Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, Republic of Korea

    Joon Ha

  6. Department of Plant Medicine, IALS, Gyeongsang National University, Jinju, Republic of Korea

    Jeung Joo Lee

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  1. Il Hwan Lee
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Correspondence to Yun-Hee Kim.

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Lee, I.H., Shim, D., Jeong, J.C. et al. Transcriptome analysis of root-knot nematode (Meloidogyne incognita)-resistant and susceptible sweetpotato cultivars. Planta 249, 431–444 (2019). https://doi.org/10.1007/s00425-018-3001-z

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