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Comparative proteomic analysis of the response of fibrous roots of nematode-resistant and -sensitive sweet potato cultivars to root-knot nematode Meloidogyne incognita

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Abstract

As a major root-knot nematode (RKN), Meloidogyne incognita causes serious losses in the yield of sweet potato (Ipomoea batatas L.). To successfully colonize the host plant, RKNs elicit changes of dramatic physiological and morphological features in the plants. The expression of several genes is regulated as the nematode establishes its feeding site. Therefore, in this study, we analyzed the proteomes in the fibrous roots of sweet potato plants by an infection of RKN to understand the effect of the infection on the plant root regions. This study revealed differences in proteomes of the RKN-resistant sweet potato cultivar Juhwangmi and RKN-sensitive cultivar Yulmi. During plant growth, Juhwangmi plants were shown to be more resistant to M. incognita than Yulmi plants. No M. incognita egg formation was observed in Juhwangmi plants, whereas 587 egg masses were formed in Yulmi plants. Differentially expressed 64 spots were confirmed by proteomic analysis using 2-D gel electrophoresis with three spots up-regulated in the two cultivars during RKN infection. Of these 64 protein spots, 20 were identified as belonging to such different functional categories as the defense response, cell structure, and energy metabolism. This study provides insight into the molecular and biochemical mechanics of the defense response and metabolism of sweet potato plant during nematode invasion. We anticipate that this study will also provide a molecular basis for useful crop breeding and the development of nematode-tolerant plants.

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Acknowledgements

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2015R1C1A1A02036323), and KRIBB Research Initiative Program (KGM5281711).

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Author notes

  1. Joon Ha and Jong Chan Won are contributed equally to this work.

Authors and Affiliations

  1. Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, South Korea

    Joon Ha & Jong Chan Won

  2. SM Biovision Co, Jinju, South Korea

    Yong Hak Jung

  3. Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan, South Korea

    Jung-Wook Yang & Hyeong-Un Lee

  4. Department of Biology Education, IALS, Gyeongsang National University, Jinju, South Korea

    Ki Jung Nam & Yun-Hee Kim

  5. Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, South Korea

    Sung-Chul Park & Jae Cheol Jeong

  6. Department of Agonomy and Medicinal Plant Resources, Gyeongnam National University of Science and Technology, Jinju, South Korea

    Shin-Woo Lee

  7. Department of Ecological Science, Kyungpook National University, Sangju, South Korea

    Dong Woon Lee

  8. Department of Agricultural Plant Science, IALS, Gyeongsang National University, Jinju, South Korea

    Jung-Sung Chung

  9. Department of Plant Medicine, IALS, Gyeongsang National University, Jinju, 660-701, South Korea

    Jeung Joo Lee

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  1. Joon Ha
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  2. Jong Chan Won
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  3. Yong Hak Jung
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  9. Shin-Woo Lee
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  10. Dong Woon Lee
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  12. Jeung Joo Lee
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Correspondence to Jeung Joo Lee or Yun-Hee Kim.

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Communicated by M. Stobiecki.

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Ha, J., Won, J.C., Jung, Y.H. et al. Comparative proteomic analysis of the response of fibrous roots of nematode-resistant and -sensitive sweet potato cultivars to root-knot nematode Meloidogyne incognita . Acta Physiol Plant 39, 262 (2017). https://doi.org/10.1007/s11738-017-2560-0

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  • DOI: https://doi.org/10.1007/s11738-017-2560-0

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