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Gene expression changes in Arabidopsis seedlings during short- to long-term exposure to 3-D clinorotation

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Abstract

Seedlings of Arabidopsis thaliana (cv. Columbia) were used to evaluate dynamic transcriptional-level genome responses to simulated microgravity condition created by 3-D clinorotation. The DNA chip data analysis showed that the plant may respond to simulated microgravity by dynamic induction (up- and down-regulations) of the responsive genes in the genome. The qRT-PCR results on the investigated genes showed that the expression patterns of the genes (molecular response) were generally similar to the physiological response patterns detected in stress-challenged plants. Expression patterns were categorized into short or continual up- or down-regulated patterns, as well as stochastic changes from short- to long-term simulated microgravity stress. The induced genes are then assumed to establish a new molecular plasticity to the newly adjusted genome status in the basic milieu of maintaining homeostasis during the process of adaptation to simulated microgravity.

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Abbreviations

dCHIP:

DNA-chip analyzer

qRT-PCR:

Quantitative real-time transcription polymerase chain reaction

3-D clinostat:

3-Dimensional clinostat

IAA:

Indole-3-acetic acid

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Authors and Affiliations

  1. Department of Genetic Engineering, Sungkyunkwan University, Suwon, 440-746, Korea

    Hyuncheol Soh & Sukchan Lee

  2. Division of Life Sciences, Mokpo National University, Muan, 534-729, Korea

    Chungkyun Auh

  3. Department of Biological Science, Chonbuk National University, Jeonju, 561-756, Korea

    Woong-Young Soh

  4. Department of Beauty and Cosmetics, College of Health and Welfare, Woosuk University, Samnye, Chonbuk, 565-701, Korea

    Kyeongsik Han

  5. Department of Biological Sciences, Silla University, Busan, 617-736, Korea

    Donggiun Kim

  6. The Institute of Life Science and Technology, Sungkyunkwan University, Suwon, 440-746, Korea

    Yong Rhee

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  1. Hyuncheol Soh
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  2. Chungkyun Auh
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  3. Woong-Young Soh
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  4. Kyeongsik Han
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  5. Donggiun Kim
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  6. Sukchan Lee
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Correspondence to Sukchan Lee or Yong Rhee.

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Soh, H., Auh, C., Soh, WY. et al. Gene expression changes in Arabidopsis seedlings during short- to long-term exposure to 3-D clinorotation. Planta 234, 255–270 (2011). https://doi.org/10.1007/s00425-011-1395-y

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