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Isolation and characterization of a rice mutant with narrow and rolled leaves

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Abstract

Appropriate leaf shape has proved to be useful in improving photosynthesis and increasing grain yield. To understand the molecular mechanism of leaf morphogenesis, we identified a rice mutant nrl1, which was characterized by a phenotype of narrow and rolled leaves. Microscopic observation showed that the mutation significantly decreased the number of vascular bundles of leaf and stem. Genetic analysis revealed that the mutation was controlled by a single nuclear-encoded recessive gene. To isolate the nrl1 gene, 756 F2 and F3 mutant individuals from a cross of the nrl1 mutant with Longtepu were used and a high-resolution physical map of the chromosomal region around the nrl1 gene was made. Finally, the gene was mapped in 16.5 kb region between marker RL21 and marker RL36 within the BAC clone OSJNBa0027H05. Cloning and sequencing of the target region from the mutant showed that there was a 58 bp deletion within the second exon of the cellulose synthase-like D4 gene (TIGR locus Os12g36890). The nrl1 mutation was rescued by transformation with the wild-type cellulose synthase-like D4 gene. Accordingly, the cellulose synthase-like D4 gene was identified as the NRL1 gene. NRL1 was transcribed in various tissues and was mainly expressed in panicles and internodes. NAL7 and SLL1 were found to be upregulated, whereas OsAGO7 were downregulated in the nrl1 mutant. These findings suggested that there might be a functional association between these genes in regulating leaf development.

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Abbreviations

CSL:

Cellulose synthase like

InDel:

Insertion–deletion

nrl1 :

Narrow and rolled leaves 1

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Acknowledgments

This project was supported by National Natural Science Foundation of China (30900790), the National High Technology Research and Development Program of China (2006AA10Z1E8) and Zhejiang Natural Science Foundation (Y307070 and 2008C22077). We are grateful to Mrs Honglan Yan (China National Rice Research Institute, China) for taking pictures for the article.

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Authors and Affiliations

  1. State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, 310006, Hangzhou, Zhejiang, China

    Chao Wu, Yaping Fu, Guocheng Hu, Huamin Si, Shihua Cheng & Wenzhen Liu

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  1. Chao Wu
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  2. Yaping Fu
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  3. Guocheng Hu
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  4. Huamin Si
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  5. Shihua Cheng
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  6. Wenzhen Liu
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Corresponding authors

Correspondence to Shihua Cheng or Wenzhen Liu.

Additional information

The sequences in this article can be found in NCBI under the following accession numbers: OsACT1 (NM_001057621), NRL1 (AK242601), SLL1 (FJ268748) NAL1 (EU093963), NAL7 (AB354302) and OsAGO7 (EF486281). The accession numbers of proteins used in the phylogeny are shown in Supplemental Data Set 1.

C. Wu, Y. Fu contributed equally to this work.

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Wu, C., Fu, Y., Hu, G. et al. Isolation and characterization of a rice mutant with narrow and rolled leaves. Planta 232, 313–324 (2010). https://doi.org/10.1007/s00425-010-1180-3

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