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Overexpression of OsCOIN, a putative cold inducible zinc finger protein, increased tolerance to chilling, salt and drought, and enhanced proline level in rice

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Abstract

Rice (Oryza sativa L.) plant is sensitive to chilling, particularly at early stages of seedling development. Here a novel cold-inducible gene, designated OsCOIN (O ryza s ativa cold-inducible), was isolated and characterized. Results showed that OsCOIN protein, a RING finger protein, was localized in both nuclear and cytoplasm membrane. OsCOIN is expressed in all rice organs and strongly induced by low temperature, ABA, salt and drought. Over-expression of OsCOIN in transgenic rice lines significantly enhanced their tolerance to cold, salt and drought, accompanied by an up-regulation of OsP5CS expression and an increase of cellular proline level.

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Acknowledgments

We thank Dr Dewang Zhou (University of Alabama at Birmingham) for critical reading and useful comments of the manuscript. This work was supported by the Major State Basic Research Program of the P.R. China (2005CB120806), the National Natural Science Foundation of China (30470866) and the State High-tech Project (2006AA10Z169), as well as innovation grants of the Chinese Academy of Sciences.

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Authors and Affiliations

  1. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Science, Beijing, 100093, China

    Kaimao Liu, Lei Wang, Yunyuan Xu, Na Chen, Qibin Ma, Fei Li & Kang Chong

  2. National Center for Plant Gene Research, Beijing, 100093, China

    Kang Chong

  3. Graduate University of the Chinese Academy of Sciences, Beijing, 100049, China

    Lei Wang, Na Chen, Qibin Ma & Fei Li

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  1. Kaimao Liu
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  2. Lei Wang
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  3. Yunyuan Xu
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  4. Na Chen
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  5. Qibin Ma
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  6. Fei Li
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  7. Kang Chong
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Correspondence to Kang Chong.

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Liu, K., Wang, L., Xu, Y. et al. Overexpression of OsCOIN, a putative cold inducible zinc finger protein, increased tolerance to chilling, salt and drought, and enhanced proline level in rice. Planta 226, 1007–1016 (2007). https://doi.org/10.1007/s00425-007-0548-5

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  • DOI: https://doi.org/10.1007/s00425-007-0548-5

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