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Evidence for intracellular spatial separation of hexokinases and fructokinases in tomato plants

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Abstract

Four hexokinase (LeHXK1–4) and four fructokinase (LeFRK1–4) genes were identified in tomato plants. Previous GFP fusion studies indicate that the gene product of LeHXK3 is associated with the mitochondria while that of LeHXK4 is located within plastids. In this study we found that the enzyme encoded by the fructokinase gene LeFRK3 is also located within plastids. The presence of LeFrk3 enzyme in plastids raises the question of the origin of fructose in these organelles. The other three FRKs enzymes, LeFrk1&2&4, are located in the cytosol. Unlike LeFrk1&2&4, the two additional HXKs, LeHxk1&2, share a common membrane anchor domain and are associated with the mitochondria similar to LeHxk3. The difference in the locations of the cytoplasmic FRK and HXK isozymes suggests that glucose phosphorylation is confined to defined special intracellular localizations while fructose phosphorylation is less confined.

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Fig. 1
Fig. 2

Abbreviations

FRK:

Fructokinase

GFP:

Green fluorescent protein

HXK:

Hexokinase

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Acknowledgments

This research was supported by research grant No. 582/01 from The Israel Science Foundation, and by research grant No. IS-3326-02C from BARD, the United States—Israel Binational Agricultural and Development Fund.

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Authors and Affiliations

  1. Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel

    Hila Damari-Weissler, Michal Kandel-Kfir, David Gidoni, Anahit Mett, Eddy Belausov & David Granot

Authors
  1. Hila Damari-Weissler
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  2. Michal Kandel-Kfir
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  3. David Gidoni
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  4. Anahit Mett
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  5. Eddy Belausov
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  6. David Granot
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Corresponding author

Correspondence to David Granot.

Additional information

Contribution from the Agriculture Research Organization, The Volcani Center, Bet Dagan, Israel, No. 126/2006 series.

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Damari-Weissler, H., Kandel-Kfir, M., Gidoni, D. et al. Evidence for intracellular spatial separation of hexokinases and fructokinases in tomato plants. Planta 224, 1495–1502 (2006). https://doi.org/10.1007/s00425-006-0387-9

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  • DOI: https://doi.org/10.1007/s00425-006-0387-9

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