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Wheat D-type cyclin Triae;CYCD2;1 regulate development of transgenic Arabidopsis plants

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Abstract

The D-type cyclin genes play key roles in plant development of Arabidopsis. To investigate the functions of cyclins in monocots, a putative ortholog of cyclin D2 genes was isolated from wheat shoot tissues, and designated as Triae;CYCD2;1. The transcripts of Triae;CYCD2;1 were primarily localized in the proliferating tissues, particularly, in shoot apical meristem and leaf primordia of wheat plants. Ectopic expression of Triae;CYCD2;1 in Arabidopsis affected plant morphology and retarded plant growth. Further examination showed that the promotion of cell division and the inhibition of cell differentiation occurred in both transgenic plants and tissues. In vitro experiments indicated that Triae;CYCD2;1 had functional roles in responding to cytokinin and auxin. Molecular analysis revealed that the transcript levels of several cell cycle-associated genes, particularly Arath;CYCD3;1, were increased in the Arabidopsis plants with the expressing Triae;CYCD2;1. The results in this study provide new information on D-type cyclin in wheat.

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Abbreviations

CaMV:

Cauliflower mosaic virus

GUS:

Beta-glucuronidase

RT-PCR:

Reverse transcriptase polymerase chain reaction

CDK:

Cyclin-dependent kinases

Rb:

Retinoblastoma-related protein

SAM:

Shoot apex meristem

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Acknowledgments

We thank Dr. Y. B. Xue for his generosity to helping us with real-time quantitative PCR (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing) and Dr. Y. Y. Xu for his helping in in situ hybridization (Institute of Botany, Chinese Academy of Sciences, Beijing). This work was supported by National Natural Science Foundation and the 863 Project of China.

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Authors and Affiliations

  1. Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, China

    Fang Wang, Sheng Nan Huo, Jing Guo & Xian Sheng Zhang

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  1. Fang Wang
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  2. Sheng Nan Huo
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  3. Jing Guo
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  4. Xian Sheng Zhang
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Correspondence to Xian Sheng Zhang.

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Wang, F., Huo, S.N., Guo, J. et al. Wheat D-type cyclin Triae;CYCD2;1 regulate development of transgenic Arabidopsis plants. Planta 224, 1129–1140 (2006). https://doi.org/10.1007/s00425-006-0294-0

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  • DOI: https://doi.org/10.1007/s00425-006-0294-0

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