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Fungal pathogen-induced changes in the antioxidant systems of leaf peroxisomes from infected tomato plants

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Abstract

Peroxisomes, being one of the main organelles where reactive oxygen species (ROS) are both generated and detoxified, have been suggested to be instrumental in redox-mediated plant cell defence against oxidative stress. We studied the involvement of tomato (Lycopersicon esculentum Mill.) leaf peroxisomes in defence response to oxidative stress generated upon Botrytis cinerea Pers. infection. The peroxisomal antioxidant potential expressed as superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6) and glutathione peroxidase (GSH-Px, EC 1.11.1.19) as well as the ascorbate-glutathione (AA-GSH) cycle activities was monitored. The initial infection-induced increase in SOD, CAT and GSH-Px indicating antioxidant defence activation was followed by a progressive inhibition concomitant with disease symptom development. Likewise, the activities of AA-GSH cycle enzymes: ascorbate peroxidase (APX, EC 1.11.1.11), monodehydroascorbate reductase (MDHAR, EC 1.6.5.4), dehydroascorbate reductase (DHAR, EC 1.8.5.1) and glutathione reductase (GR, EC 1.6.4.2) as well as ascorbate and glutathione concentrations and redox ratios were significantly decreased. However, the rate and timing of these events differed. Our results indicate that B. cinerea triggers significant changes in the peroxisomal antioxidant system leading to a collapse of the protective mechanism at advanced stage of infection. These changes appear to be partly the effect of pathogen-promoted leaf senescence.

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Abbreviations

AA:

Reduced ascorbate

APX:

Ascorbate peroxidase

CAT:

Catalase

DHA:

Dehydroascorbate

DHAR:

Dehydroascorbate reductase

GR:

Glutathione reductase

GSH:

Reduced glutathione

GSH-Px:

Glutathione peroxidase

GSSG:

Oxidised glutathione

MDHAR:

Monodehydroascorbate reductase

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

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Acknowledgements

This work has been partly supported by University of Łódź Grants No 505/429 and 505/430.

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  1. Department of Plant Physiology and Biochemistry, University of Łódź, 90-237 Łódź, Banacha, 12/16, Poland

    Elźbieta Kużniak & Maria Skłodowska

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  1. Elźbieta Kużniak
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  2. Maria Skłodowska
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Correspondence to Elźbieta Kużniak.

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Kużniak, E., Skłodowska, M. Fungal pathogen-induced changes in the antioxidant systems of leaf peroxisomes from infected tomato plants. Planta 222, 192–200 (2005). https://doi.org/10.1007/s00425-005-1514-8

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