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Oxalate contributes to the resistance of Gaillardia grandiflora and Lupinus sericeus to a phytotoxin produced by Centaurea maculosa

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Abstract

Centaurea maculosa Lam. is a noxious weed in western North America that produces a phytotoxin, (±)-catechin, which is thought to contribute to its invasiveness. Areas invaded by C. maculosa often result in monocultures of the weed, however; in some areas, North American natives stand their ground against C. maculosa and show varying degrees of resistance to its phytotoxin. Two of these resistant native species, Lupinus sericeus Pursh and Gaillardia grandiflora Van Houtte, were found to secrete increased amounts of oxalate in response to catechin exposure. Mechanistically, we found that oxalate works exogenously by blocking generation of reactive oxygen species in susceptible plants and reducing oxidative damage generated in response to catechin. Furthermore, field experiments show that L. sericeus indirectly facilitates native grasses in grasslands invaded by C. maculosa, and this facilitation can be correlated with the presence of oxalate in soil. Addition of exogenous oxalate to native grasses and Arabidopsis thaliana (L.) Heynh grown in vitro alleviated the phytotoxic effects of catechin, supporting the field experiments and suggesting that root-secreted oxalate may also act as a chemical facilitator for plant species that do not secrete the compound.

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Abbreviations

HPLC:

High pressure liquid chromatography

ROS:

Reactive oxygen species

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Acknowledgements

This work was supported by grants from USDA-WRIPM (grant no. 2003-05060, J.M.V.), National Science Foundation- (grant no. NSF-IBN 0335203, J.M.V.), U.S. Department of Defense SERDP (CS1388 to J.M.V. and R.M.C.) and Invasive Weeds Initiative of the State of Colorado (J.M.V. and F.R.S.). JMV is an NSF-CAREER faculty fellow (grant no. MCB 093014).

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Authors and Affiliations

  1. Department of Horticulture and Landscape Architecture, Graduate Program in Cell and Molecular Biology, Colorado State University, 1173 Horticulture and Landscape Architecture, Fort Collins, CO, 80523, USA

    Tiffany L. Weir & Jorge M. Vivanco

  2. Center for Rhizosphere Biology, Colorado State University, Fort Collins, CO, 80523, USA

    Tiffany L. Weir, Valerie J. Stull & Jorge M. Vivanco

  3. Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, 80523, USA

    Suresh Bhamidi

  4. Department of Chemistry, Colorado State University, Fort Collins, CO, 80523, USA

    Valerie J. Stull & Frank R. Stermitz

  5. Department of Plant and Soil, Delaware Biotechnology Institute, University of Delaware, Newark, DE, 19702, USA

    Harsh Pal Bais

  6. The Division of Biological Sciences, The University of Montana, Missoula, MT, 59812, USA

    Ragan M. Callaway, Giles C. Thelen & Wendy M. Ridenour

Authors
  1. Tiffany L. Weir
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  2. Harsh Pal Bais
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  3. Valerie J. Stull
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  4. Ragan M. Callaway
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  8. Frank R. Stermitz
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  9. Jorge M. Vivanco
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Correspondence to Jorge M. Vivanco.

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Weir, T.L., Bais, H.P., Stull, V.J. et al. Oxalate contributes to the resistance of Gaillardia grandiflora and Lupinus sericeus to a phytotoxin produced by Centaurea maculosa . Planta 223, 785–795 (2006). https://doi.org/10.1007/s00425-005-0192-x

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  • DOI: https://doi.org/10.1007/s00425-005-0192-x

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