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SKS6, a multicopper oxidase-like gene, participates in cotyledon vascular patterning during Arabidopsis thaliana development

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Abstract

SKU5-Similar 6 (SKS6) is a one of a large gene family of 19 members in Arabidopsis thaliana (L.) Heynh that encode multicopper oxidase-like proteins that are related to ferroxidases, ascorbate oxidases and laccases. Only one member of the family has been previously studied; Skewed5 (SKU5) is involved in the control of root growth. The encoded SKS6 protein, like SKU5 appears to lack a functional copper-binding site and is most closely related to Bp10 from Brassica napus and Ntp303 from Nicotiana tobacum. The SKS6 promoter contains many putative regulatory sites and differential expression of an SKS6::GUS reporter gene revealed selective induction in several seedling tissues including guard cells, root cortex cells, and leaf margin hydathodes. It was also expressed later in flower development in flower primordia, ovules, and the abscission zones of seeds and siliques. Furthermore, SKS6 was upregulated in roots in response to treatment of seedlings with the hormones abscisic acid, indole-3 acetic acid, 2,4-dichlorophenoxyacetic acid and aminocyclopropane-1-carboxylate. A loss-of function sks6-1 T-DNA insertion allele revealed that cotyledon vascular patterning is affected in the mutant, suggesting a role for the protein in metabolism of nutrients or hormones in the hydathodes, the sites of auxin synthesis and chemical recycling.

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Abbreviations

2, 4-D:

2,4-dichlorophenoxyacetic acid

ABA:

Abscisic acid

ABRC:

Arabidopsis Biological Resource Center

ACC:

Aminocyclopropane-1-carboxylate

AO:

Ascorbate oxidase

BA N6 :

Benzyladenine

GFP:

Green flourescent protein

GUS:

β-glucuronidase

IAA:

Indole-3 acetic acid

LB:

Luria Broth

M&S:

Murashige and Skoog

MES:

2-[N-morpholino] ethane sulfonic acid

dNTP:

Deoxynucleotide triphosphate

PCR:

Polymerase chain reaction

RT-PCR:

Reverse transcription-polymerase chain reaction

Skewed5:

SKU5

SKS6:

SKU5-Similar 6

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Acknowledgements

This work was supported by Kansas State University Agricultural Experiment Station project KS517 and the Terry C. Johnson Center for Basic Cancer Research. JJ was supported by Kansas State University Plant Biotechnology Center.

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  1. Division of Biology, Kansas State University, Ackert Hall, Manhattan, KS, 66506, USA

    Jolanta Jacobs & Judith L. Roe

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  1. Jolanta Jacobs
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Correspondence to Judith L. Roe.

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Jacobs, J., Roe, J.L. SKS6, a multicopper oxidase-like gene, participates in cotyledon vascular patterning during Arabidopsis thaliana development. Planta 222, 652–666 (2005). https://doi.org/10.1007/s00425-005-0012-3

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