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Identification and fine mapping of a mutant gene for palealess spikelet in rice

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Abstract

In grass, the evolutionary relationship between lemma and palea, and their relationship to the flower organs in dicots have been variously interpreted and wildely debated. In the present study, we carried out morphological and genetic analysis of a palealess mutant (pal) from rice (Oryza sativa L.), and fine mapping the gene responsible for the mutated trait. Together, our findings indicate that the palea is replaced by two leaf-like structures in the pal flowers, and this trait is controlled by one recessive gene, termed palealess1 (pal1). With a large F2 segregating population, the pal1 gene was finally mapped into a physical region of 35 kb. Our results also suggest that the lemma and palea of rice are not homologous organs, palea is likely evolutionarily equivalent to the eudicot sepal, and the pal1 should be an A function gene for rice floral organ identity.

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Fig. 1
Fig. 2a–e
Fig. 3a–l
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Fig. 6a–e
Fig. 7a–c

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Abbreviations

pal :

Palealess mutant

pal1 :

Palealess1

PAL1 :

PALEALESS1

SEM:

Scanning electron microscopy

EREBP:

Ethylene-responsive element-binding protein

AP2-like:

APETALA2-like

ap1-1:

Apetala1-1

ap2-1:

Apetala2-1

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Acknowledgements

We would like to thank Prof. K.D. Zhou (Rice Research Institute of Sichuan Agriculture University, China) for providing parental materials, Dr X.Q. Wang (Institute of Genetics and Developmental Biology, CAS, China) for technical assistance in microscopy, and Prof. W. Xue (Institute of Biophysics, CAS, China) for SEM analysis. This work was supported by a grant from the National Natural Science Foundation of China (30370797).

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Authors and Affiliations

  1. Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing, 100101, China

    Qiong Luo, Xianfeng Zhao, Hongai Xia, Wenxue Zhai, Jichen Xu & Lihuang Zhu

  2. Rice Research Institute of Sichuan Agriculture University, Wenjiang, 611130, China

    Kaida Zhou, Xianjun Wu & Hongsong Yang

  3. Faculty of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China

    Qianchun Zeng

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  1. Qiong Luo
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  2. Kaida Zhou
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Correspondence to Lihuang Zhu.

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Luo, Q., Zhou, K., Zhao, X. et al. Identification and fine mapping of a mutant gene for palealess spikelet in rice. Planta 221, 222–230 (2005). https://doi.org/10.1007/s00425-004-1438-8

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