Abstract
Maximum velocity of shortening, V o, was measured by the method of Edman [J Physiol (Lond) 291:143–159, 1979] on extensor digitorum longus muscles of a mouse in vitro at 20°C. Blockers of nitric oxide synthase, 10 mM nitro-l-arginine or 1 mM 7-nitroindazole, reduced V o by 18% and 22%, respectively. On removal of the inhibitor, V o returned to the control value. It was found that 10 mM nitro-d-arginine, an enantiomer of nitro-l-arginine inactive against nitric oxide synthase, did not affect V o. A donor of nitric oxide, 0.1 mM nitroprusside, increased V o by 15%. It removed the inhibition caused by nitro-l-arginine. Another donor of nitric oxide, 1 µM (±)-S-nitroso-N-acetylpenicillamine (SNAP), increased V o by 8%. An inhibitor of cGMP synthase, 0.01 mM Ly-83583, decreased V o by 18%. An analogue of cGMP, 0.1 mM 8-bromo-cGMP, increased V o by 17%. A general inhibitor of phosphodiesterases, 0.02 mM 3-isobutyl-1-methylxanthine (IBMX), increased V o by 17%. An inhibitor specific of cGMP phosphodiesterase, 0.01 mM dipyridamole, increased V o by 8%. The maximal isometric force (F 0) was not modified by the drugs, except by 7-nitroindazole and Ly-83583, which depressed F 0 by 12%. The cGMP level in tetanized muscles decreased by 12–27% in the presence of blockers of nitric oxide synthase. We conclude that the level of intracellular nitric oxide modulates V o through thecGMP pathway.
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Received: 16 February 1998 / Received after revision: 18 May 1998 / Accepted: 18 June 1998
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Maréchal, G., Beckers-Bleukx, G. Effect of nitric oxide on the maximal velocity of shortening of a mouse skeletal muscle. Pflügers Arch 436, 906–913 (1998). https://doi.org/10.1007/s004240050722
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DOI: https://doi.org/10.1007/s004240050722