Abstract
Among G-proteins, Gh is unique in structural differences in the GTP-binding domain and possessing transglutaminase activity. We have studied the role of G protein in modulation of large conductance Ca2+-activated K+ (Maxi-K+) channel by the inside-out mode of patch clamp in smooth muscle cells from superior mesenteric artery of the rabbit. When the non-hydrolyzable GTP analogue, GTPγS, was applied, the channel activity was increased about 2.5-fold. Addition of GDPβS resulted in reversal of the GTPγS effect. When the Gαh7 antibody was applied, the GTPγS-stimulated channel activity was significantly inhibited to control level, suggesting that Gαh is involved in activation of the Maxi-K+ channel in smooth muscle cells.
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Received: 23 September 1996 / Received after revision: 26 November 1996 / Accepted: 3 December 1996
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Lee, MY., Chung, S., Bang, HW. et al. Modulation of large conductance Ca2+-activated K+ channel by Gαh (transglutaminase II) in the vascular smooth muscle cell. Pflügers Arch 433, 671–673 (1997). https://doi.org/10.1007/s004240050330
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DOI: https://doi.org/10.1007/s004240050330