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Effect of temperature on FAD and NADH-derived signals and neurometabolic coupling in the mouse auditory and motor cortex

  • Neuroscience
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Abstract

Tight coupling of neuronal metabolism to synaptic activity is critical to ensure that the supply of metabolic substrates meets the demands of neuronal signaling. Given the impact of temperature on metabolism, and the wide fluctuations of brain temperature observed during clinical hypothermia, we examined the effect of temperature on neurometabolic coupling. Intrinsic fluorescence signals of the oxidized form of flavin adenine dinucleotide (FAD) and the reduced form of nicotinamide adenine dinucleotide (NADH), and their ratios, were measured to assess neural metabolic state and local field potentials were recorded to measure synaptic activity in the mouse brain. Brain slice preparations were used to remove the potential impacts of blood flow. Tight coupling between metabolic signals and local field potential amplitudes was observed at a range of temperatures below 29 °C. However, above 29 °C, the metabolic and synaptic signatures diverged such that FAD signals were diminished, but local field potentials retained their amplitude. It was also observed that the declines in the FAD signals seen at high temperatures (and hence the decoupling between synaptic and metabolic events) are driven by low FAD availability at high temperatures. These data suggest that neurometabolic coupling, thought to be critical for ensuring the metabolic health of the brain, may show temperature dependence, and is related to temperature-dependent changes in FAD supplies.

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Acknowledgements

The Carle Neuroscience Institute supported the work. D.A.L. was supported by DC013073. The authors thank Dr. Eugene Kiyatkin (NIH), Dr. Robert Gennis (University of Illinois), Dr. Sanjiv Sinha (University of Illinois), Dr. Naoum Issa (University of Chicago), Dr. Jan Ramirez (Seattle Children’s Hospital), and Dr. Alfredo Garcia (Seattle Children’s Hospital) for their suggestions.

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Authors and Affiliations

  1. Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA

    Baher A. Ibrahim, Bethany Bucci & Daniel A. Llano

  2. The Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 North Mathews Avenue, Urbana, IL, 61801, USA

    Baher A. Ibrahim, Bethany Bucci, Kush Paul & Daniel A. Llano

  3. College of Medicine, University of Illinois at Urbana-Champaign, Urbana, IL, USA

    Huan Wang & Daniel A. Llano

  4. Carle Neuroscience Institute, Carle Foundation Hospital, Urbana, IL, USA

    Huan Wang & Daniel A. Llano

  5. Re3 Innovative Neuroscience Institute, P.L.L.C, Sarasota, FL, USA

    Huan Wang

  6. Re3 Stem Cell and Healing Institute, P.L.L.C, Sarasota, FL, USA

    Huan Wang

  7. Neuroscience Program, University of Illinois at Urbana-Champaign, Urbana, IL, USA

    Alexandria M. H. Lesicko & Daniel A. Llano

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  1. Baher A. Ibrahim
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  2. Huan Wang
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  3. Alexandria M. H. Lesicko
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Correspondence to Daniel A. Llano.

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Ibrahim, B.A., Wang, H., Lesicko, A.M.H. et al. Effect of temperature on FAD and NADH-derived signals and neurometabolic coupling in the mouse auditory and motor cortex. Pflugers Arch - Eur J Physiol 469, 1631–1649 (2017). https://doi.org/10.1007/s00424-017-2037-4

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  • DOI: https://doi.org/10.1007/s00424-017-2037-4

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