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Post-hypoxic cellular disintegration in glycine-preserved renal tubules is attenuated by hydroxyl radical scavengers and iron chelators

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Abstract

Background

Cellular stress during reoxygenation is a common phenomenon in solid organ transplantation and is characterized by production of reactive oxygen species. Herein, we studied in isolated tubular segments of rat kidney cortex the impact of oxygen radical scavengers and an iron chelator on post-hypoxic recovery.

Methods

Tubules, suspended in Ringer’s solution containing 5 mM glycine, underwent 30 min hypoxia and 60 min reoxygenation. Untreated tubules served as controls. Hypoxia–reoxygenation injury was measured by membrane leakage, lipid peroxidation and cellular functions. In hypoxia–reoxygenated-isolated tubular segments, protective effects of different scavengers and of the iron chelator deferoxamine on hypoxia–reoxygenation injury were analyzed.

Results

Scavengers protected isolated tubular segments from hypoxia–reoxygenation-induced cellular disintegration and dysfunction. Deferoxamine was found to exert the most distinct protection. It was further found to exert a dose-dependent protection on hypoxia–reoxygenation damage in isolated tubular segments, which was critically mediated by chelating tissue and bond iron.

Conclusions

Our data demonstrate that radical scavengers effectively protect from hypoxia–reoxygenation injury in isolated tubular segments and that the iron chelator deferoxamine is especially a potent inhibitor of iron ion-mediated hypoxia–reoxygenation damage. Thus, inclusion of this iron chelator in organ storage solutions might improve post-transplant organ function and protect from reperfusion injury.

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Authors and Affiliations

  1. Department of General, Visceral, Vascular and Pediatric Surgery, University of Saarland, 66421, Homburg, Saarland, Germany

    Mohammed R. Moussavian, Jan E. Slotta, Otto Kollmar & Martin K. Schilling

  2. Institute for Clinical and Experimental Surgery, University of Saarland, 66421, Homburg, Saarland, Germany

    Michael D. Menger

  3. Department of Physiology, University of Kiel, Olshausenstrasse 40, 24098, Kiel, Germany

    Gernot Gronow

Authors
  1. Mohammed R. Moussavian
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  2. Jan E. Slotta
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  3. Otto Kollmar
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  4. Michael D. Menger
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  5. Gernot Gronow
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  6. Martin K. Schilling
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Corresponding author

Correspondence to Martin K. Schilling.

Additional information

German Society of Surgery, Surgical Forum 2008, Best of Abstracts.

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Moussavian, M.R., Slotta, J.E., Kollmar, O. et al. Post-hypoxic cellular disintegration in glycine-preserved renal tubules is attenuated by hydroxyl radical scavengers and iron chelators. Langenbecks Arch Surg 393, 303–310 (2008). https://doi.org/10.1007/s00423-008-0287-5

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  • DOI: https://doi.org/10.1007/s00423-008-0287-5

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