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Protective skeletal muscle arteriolar vasomotion during critical perfusion conditions of osteomyocutaneous flaps is not mediated by nitric oxide and endothelins

  • Musculoskeletal Soft Tissue Conditioning
  • Published:
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Abstract

Background

After flap surgery, vasomotion, defined as oscillation of the arteriolar diameter, may protect tissue during critical perfusion conditions. The mechanisms that regulate vasomotion are still unclear; therefore, we studied the incidence of vasomotion in peripheral tissue and whether nitric oxide or endothelins are involved in regulation of vasomotion.

Materials and methods

In Sprague–Dawley rats, an osteomyocutaneous flap was prepared. To induce critical perfusion conditions, we reduced arterial blood flow supplying the flap to 0.15 ml/min. Seven animals received NG-nitro-l-arginine methyl ester (L-NAME), a nitric oxide-synthase inhibitor, and six animals bosentan, an endothelin A/B receptor antagonist. Microcirculation of muscle, skin, subcutis and periosteum was assessed by intravital microscopy before and after drug application.

Results

In all animals, reduction of arterial blood supply induced arteriolar vasomotion in muscle (100%), but not in periosteum, subcutis and skin. Vasomotion was found to be affected by neither L-NAME (frequency 2.6±0.2 versus 2.4±0.2 cycles/min; amplitude 67±19 versus 55±20%; share of dilation period in vasomotion cycle 59±2 versus 58±3%) nor bosentan (1.8±0.1 versus 1.7±0.1 cycles/min; 60±10 versus 64±6%; 50±2 versus 53±1%).

Conclusions

Our study indicates that during critical perfusion conditions, arteriolar vasomotion develops only in muscle, not in skin, subcutis and periosteum, and that nitric oxide and endothelins are not involved in the regulation of this protective vascular response.

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Acknowledgement

This study was supported by a grant from the Deutsche Forschungsgemeinschaft (Me 900/1–3 and 1–4).

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, University of Saarland, 66421 Homburg/Saar, Germany

    Martin Rücker & Wolfgang J. Spitzer

  2. Institute for Clinical and Experimental Surgery, University of Saarland, Homburg/Saar, Germany

    Martin Rücker, Oliver Strobel, Brigitte Vollmar & Michael D. Menger

Authors
  1. Martin Rücker
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  2. Oliver Strobel
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  3. Brigitte Vollmar
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  4. Wolfgang J. Spitzer
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  5. Michael D. Menger
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Corresponding author

Correspondence to Martin Rücker.

Additional information

Part of the material was presented at the International Symposium "Significance of Musculo-Skeletal Soft Tissue on Pre-Operative Planning, Surgery and Healing", 13–14 February 2003, Berlin, Germany

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Rücker, M., Strobel, O., Vollmar, B. et al. Protective skeletal muscle arteriolar vasomotion during critical perfusion conditions of osteomyocutaneous flaps is not mediated by nitric oxide and endothelins. Langenbecks Arch Surg 388, 339–343 (2003). https://doi.org/10.1007/s00423-003-0389-z

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  • DOI: https://doi.org/10.1007/s00423-003-0389-z

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