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Daily regulation of serum and urinary hepcidin is not influenced by submaximal cycling exercise in humans with normal iron metabolism

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Abstract

Hepcidin and hemojuvelin (HJV) are two critical regulators of iron metabolism as indicated by the development of major iron overload associated to mutations in hepcidin and HJV genes. Hepcidin and HJV are highly expressed in liver and muscles, respectively. Intensive muscular exercise has been reported to modify serum iron parameters and to increase hepcidinuria. The present study aimed at evaluating the potential impact of low intensity muscle exercise on iron metabolism and on hepcidin, its key regulator. Fourteen normal volunteers underwent submaximal cycling-based exercise in a crossover design and various iron parameters, including serum and urinary hepcidin, were serially studied. The results demonstrated that submaximal ergocycle endurance exercise did not modulate hepcidin. This study also indicated that hepcidinuria did not show any daily variation whereas serum hepcidin did. The findings, by demonstrating that hepcidin concentrations are not influenced by submaximal cycling exercise, may have implications for hepcidin sampling in medical practice.

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Acknowledgments

This work was supported by COREC (Comité de Recherche Clinique) 2005 (University Hospital, Rennes), Association Fer et Foie, and by European Contract EuroIron1 (STREP No. 037296).

Conflict of interest statement

Mark Westerman is Co-founder and President of Intrinsic Life Sciences. Tomas Ganz and Elizabeta Nemeth are Co-founders and Members of the Advisory Board for Intrinsic Life Sciences. Olbina Gordana is a Senior Scientist from Intrinsic Life Sciences.

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Author notes

  1. Marie-Bérengère Troadec

    Present address: Jerry Kaplan Laboratory, Department of Pathology, University of Utah, Salt Lake City, UT, 84132, USA

Authors and Affiliations

  1. Liver Disease Unit, Inserm U-522, IFR 140, University Hospital Pontchaillou, Rennes, France

    Marie-Bérengère Troadec, Fabrice Lainé, Martine Ropert, Olivier Loréal & Pierre Brissot

  2. URU Biothérapies Innovantes, Faculté de Médecine, Université de Rennes 1 Laboratoire de cytogénétique et biologie cellulaire, University Hospital Pontchaillou, Rennes, France

    Florian Cabillic

  3. Center for Clinical Investigation, Inserm0203, University Hospital Pontchaillou, Rennes, France

    Fabrice Lainé, Michèle Perrin & Jeff Morcet

  4. Laboratory of Physiology, University Hospital Pontchaillou, Rennes, France

    Vincent Daniel

  5. Sport Medicine Unit, University Hospital Pontchaillou, Rennes, France

    Vincent Daniel & Pierre Rochcongar

  6. Intrinsic LifeSciences, LLC, La Jolla, CA, USA

    Gordana Olbina, Mark Westerman, Elizabeta Nemeth & Tomas Ganz

  7. David Geffen School of Medicine, University of California, Los Angeles, USA

    Elizabeta Nemeth & Tomas Ganz

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  1. Marie-Bérengère Troadec
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Correspondence to Marie-Bérengère Troadec.

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Troadec, MB., Lainé, F., Daniel, V. et al. Daily regulation of serum and urinary hepcidin is not influenced by submaximal cycling exercise in humans with normal iron metabolism. Eur J Appl Physiol 106, 435–443 (2009). https://doi.org/10.1007/s00421-009-1031-8

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