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Analysis of aquaporin expression in liver with a focus on hepatocytes

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Abstract

A deeper understanding of aquaporins (AQPs) expression and transcriptional regulation will provide useful information for liver pathophysiology. We established a complete AQPs mRNA expression profile in human and mouse liver, as well as protein localization of expressed AQPs. Additionally, the modulation of AQPs mRNA levels in response to various agents was determined in human HuH7 cells and in primary culture of mouse hepatocytes. AQP1, AQP3, AQP7, AQP8, and AQP9 mRNA and protein expressions were detected in human liver, while only AQP6 and AQP11 mRNAs were detected. We reported for the first time the localization of AQP3 in Kupffer cells, AQP7 in hepatocytes and endothelial cells, and AQP9 in cholangiocytes. In addition, we confirmed the localization of AQP1 in endothelial cells, and of AQP8 and AQP9 in hepatocytes. On HuH7 cells, we reported the presence of AQP4 mRNA, confirmed the presence of AQP3, AQP7, and AQP11 mRNAs, but not of AQP8 mRNA. On primary culture of murine hepatocytes, AQP1 and AQP7 mRNAs were identified, while the presence of AQP3, AQP8, AQP9, and AQP11 mRNAs was confirmed. At the protein level, murine endothelial liver cells expressed AQP1 and AQP9, while hepatocytes expressed AQP3, AQP7, AQP8, and AQP9, and macrophages expressed AQP3. Dexamethasone, forskolin, AICAR, rosiglitazone, octanoylated, and non-octanoylated ghrelin regulated some AQP expression in primary culture of murine hepatocytes and human HuH7 cells. Additional studies will be required to further assess the role of AQPs expression in human and murine liver and understand the transcriptional regulation of AQPs in hepatocytes under pathophysiological conditions.

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Abbreviations

AICAR:

Aminoimidazole carboxamide ribonucleotide

AMPK:

5′ Adenosine monophosphate-activated protein kinase

AQP:

Aquaporin

cAMP:

Cyclic cyclic adenosine monophosphate

FK:

Forskolin

GNO:

Non-octanoylated ghrelin

GO:

Octanoyalted ghrelin

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Acknowledgments

This work was supported by Grant 3.4543.10 from the Fund for Medical Scientific Research (FRSM) (Belgium), the Defay Fund, and David and Alice van Buuren Foundation. A.Z.-S. was a recipient of a FNRS fellowship (FNRS, Belgium).

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    Authors and Affiliations

    1. Laboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles, Brussels, Belgium

      Françoise Gregoire, Amal Zerrad-Saadi, Myrna Virreira, Nargis Bolaky, Valérie Delforge, Jason Perret & Christine Delporte

    2. Digestive Oncology Department, Erasme Hospital, Brussels, Belgium

      Valério Lucidi & Vincent Donckier

    3. Gastroenterology Department, Erasme Hospital, Brussels, Belgium

      Arnaud Lemmers & Jacques Devière

    4. Anatomopathology Department, Erasme Hospital, Brussels, Belgium

      Pieter Demetter

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    1. Françoise Gregoire
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    Correspondence to Christine Delporte.

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    All procedures performed in studies involving human participants were in accordance with the ethical standards of the Ethical Committee of the Erasme Hospital and with the Declaration of Helsinki and its later amendments or comparable ethical standards. All applicable national and institutional guidelines for the care and use of animals were followed.

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    Françoise Gregoire, Valério Lucidi, and Christine Delporte have contributed equally to the work.

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    Gregoire, F., Lucidi, V., Zerrad-Saadi, A. et al. Analysis of aquaporin expression in liver with a focus on hepatocytes. Histochem Cell Biol 144, 347–363 (2015). https://doi.org/10.1007/s00418-015-1341-3

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