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Cancer-associated fibroblasts are not formed from cancer cells by epithelial-to-mesenchymal transition in nu/nu mice

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Abstract

Cancer-associated fibroblasts are bioactive elements influencing the biological properties of malignant tumors. Their origin from different cell types has been established, and the possibility of their formation by epithelial-to-mesenchymal transition from cancer cells is under debate. This study shows that human cancer cells grafted to nu/nu mice induced formation of tumor stroma with the presence of typical smooth muscle actin-containing cancer-associated fibroblasts. These cells seem to be of the host origin because they are not recognized by an antibody specific for human vimentin, as was also verified in vitro. These results suggest that cancer-associated stromal fibroblasts are not formed by epithelial-to-mesenchymal transition from cancer cells.

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Acknowledgments

This study was supported by the Grant Agency of the Czech Republic, projects Nos. 13-20293S and P301/12/1254, by the grant of Grant Agency of Ministry of Health of the Czech Republic (NT13488), by the Charles University projects PRVOUK-27, UNCE 204013 and by the Specific University Research (SVV). Authors are also grateful to EC projects: BIOCEV (Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University in Vestec -CZ.1.05/1.1.00/02.0109—from the European Regional Development Fund) and the ITN network GLYCOPHARM (Contract No. 317297) for support. Authors are grateful to Marie Jindráková, Radana Kavková, Helena Mišurcová and Pavlína Jungrová for excellent technical assistance.

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Authors and Affiliations

  1. First Faculty of Medicine, Institute of Anatomy, Charles University, U nemocnice 3, 128 00, Prague 2, Czech Republic

    Barbora Dvořánková, Karel Smetana Jr., Jan Kučera, Rosana Mateu & Pavol Szabo

  2. Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Prague, Czech Republic

    Blanka Říhová

  3. Department of Dermatovenereology, First Faculty of Medicine, Charles University, Prague, Czech Republic

    Jan Kučera

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  1. Barbora Dvořánková
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  2. Karel Smetana Jr.
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  3. Blanka Říhová
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  5. Rosana Mateu
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  6. Pavol Szabo
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Correspondence to Karel Smetana Jr..

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Dvořánková, B., Smetana, K., Říhová, B. et al. Cancer-associated fibroblasts are not formed from cancer cells by epithelial-to-mesenchymal transition in nu/nu mice. Histochem Cell Biol 143, 463–469 (2015). https://doi.org/10.1007/s00418-014-1293-z

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