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Immunohistochemistry, glycosylation and immunosuppression of glycodelin in human ovarian cancer

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Abstract

Glycodelins (Gds) are glycoproteins with a gender specific glycosylation. Glycodelin A (GdA) is primarily produced in endometrial and decidual tissue and secreted to amniotic fluid. Glycodelins were also identified in several cancer types, including serous ovarian cancer. Gds act as a T-cell inhibitor and are involved in inactivation of human monocytes. With a Gd peptide antibody, derived from a 15 amino acid sequence of human Gd and in situ hybridization experiments, the expression of Gd in serous, mucinous, endometrioid and clear cell ovarian tumors was identified. In contrast to former investigations with antibodies against GdA, a positive immunohistochemical reaction for Gd was observed in all forms of epithelium ovarian cancer. These results were confirmed with in situ hybridization. In addition, Gd is expressed in granulose cell tumors, a non-epithelial form of ovarian cancer. Furthermore, Gd was purified from ascites fluid of ovarian cancer patients. Ascites Gd showed significant differences in its structure of sialyl Lewis-type oligosaccharides compared to GdA. Additionally, ascites Gd inhibits IL-2 stimulated proliferation of peripheral blood leucocytes and inhibits adhesion of SLeX-positive cells to E-selectin. Therefore, Gd could act as an inhibitor of lymphocyte activation and/or adhesion in ovarian cancer.

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Acknowledgments

We thank Dr. U. Karsten (NEMOD Biotherapeutics, Berlin, Germany) for providing the monoclonal antibodies used in this study, S. Kunze (1st Department of Obstetrics and Gynecology Ludwig Maximilians University of Munich, Munich, Germany), D.U. Richter (Department of Obstetrics and Gynecology, University of Rostock, Rostock, Germany) as well as J. Adolph (Institute of Pathology, Ernst Moritz Arndt University of Greifswald, Greifswald, Germany) for excellent technical assistance. Furthermore, we are grateful to Dr. R. Jarchow (Computing Center, University of Greifswald, Germany) for his support and to T. Shaw (former journalist of the “Daily Telegraph”) for the evaluation of English language.

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Authors and Affiliations

  1. Ist Department of Obstetrics and Gynecology, Ludwig-Maximilians-University of Munich, Maistrasse 11, 80337, Munich, Germany

    Udo Jeschke, Ioannis Mylonas, Christoph Scholz, Wolfgang Janni, Christina Kuhn & Klaus Friese

  2. Institute of Pathophysiology, Ernst Moritz Arndt University of Greifswald, Karlsburg, Germany

    Christiane Kunert-Keil

  3. Glycotope GmbH, Berlin, Germany

    Renate Stahn

  4. Institute of Pathology, Ernst Moritz Arndt University of Greifswald, Greifswald, Germany

    Eike Schröder

  5. Institute of Pathology, Ludwig-Maximilians-University of Munich, Munich, Germany

    Doris Mayr

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  1. Udo Jeschke
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Correspondence to Ioannis Mylonas.

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U. Jeschke, I. Mylonas and C. Kunert-Keil contributed equally to this work.

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Jeschke, U., Mylonas, I., Kunert-Keil, C. et al. Immunohistochemistry, glycosylation and immunosuppression of glycodelin in human ovarian cancer. Histochem Cell Biol 131, 283–295 (2009). https://doi.org/10.1007/s00418-008-0510-z

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