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Immunolocalization of glycodelin in the genital tract of rats

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Abstract

Glycodelin, also known as placental protein 14 has been predominantly localized to organs of the human genital tract. Unfortunately the physiological role of glycodelin is largely unknown since it depends on limited availability of tissues. Therefore, a suitable animal model to study the role of glycodelin would be desirable. Previously, it was shown that glycodelin mRNA is expressed in the genital tract of male and female rats. In the present study, we demonstrate the expression of glycodelin protein in male and female rats by immunohistochemistry and Western blot analysis. For this purpose a polyclonal antibody was generated against glycodelin peptide. In female rats, glycodelin was found in the epithelial gland cells of the uterus, epithelial cells of the fallopian tube as well as in corpora lutea, interstitial and theca cells of the ovary. Glycodelin was distributed in all epithelial cells of the epididymis and the seminal vesicle. In the seminiferous epithelium, glycodelin was seen in all developmental stages of spermatogonia and spermatocytes and in Sertoli cells. Whereas in the rat male reproductive tract glycodelin expression is slightly different from human or primate tissues, in organs of the rat female genital tract glycodelin expression is similar to humans and primates.

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References

  • SC Bell JO Drife (1989) ArticleTitleSecretory proteins of the endometrium - potential markers for endometrial dysfunction Baillieres Clin Obstet Gynaecol 3 271–291 Occurrence Handle2482150 Occurrence Handle1:STN:280:DyaK3c7isFektg%3D%3D

    PubMed  CAS  Google Scholar 

  • SC Bell SR Patel MW Hales PH Kirwan JO Drife (1985) ArticleTitleImmunochemical detection and characterization of pregnancy-associated endometrial α1- and α2-globulins secreted by human endometrium and decidua J Reprod Fertil 74 261–270 Occurrence Handle2410613 Occurrence Handle1:CAS:528:DyaL2MXltF2jtLs%3D Occurrence Handle10.1530/jrf.0.0740261

    Article  PubMed  CAS  Google Scholar 

  • H Bohn W Kraus W Winckler (1982) New soluble placental tissue proteins: their isolation characterization localization and quantification. A Klopper (Eds) Immunology of Human Placental Proteins Praeger Publishers Praeger 67–82

    Google Scholar 

  • A Chryssikopoulos T Mantzavinos N Kanakas E Karagouni E Dotsika PA Zourlas (1996) ArticleTitleCorrelation of serum and follicular fluid concentrations of placental protein 14 and CA-125 in in vitro fertilization-embryo transfer patients Fertil Steril 66 599–603 Occurrence Handle8816623 Occurrence Handle1:STN:280:DyaK28vhtlCluw%3D%3D

    PubMed  CAS  Google Scholar 

  • AT Fazleabas KM Donnelly S Hild-Petito HM Hausermann HG Verhage (1997) ArticleTitleSecretory proteins of the baboon (Papio anubis) endometrium: regulation during the menstrual cycle and early pregnancy Hum Reprod Update 3 553–559 Occurrence Handle9584945 Occurrence Handle1:CAS:528:DyaK1MXltVWmur0%3D

    PubMed  CAS  Google Scholar 

  • MD Ganfornina G Gutierrez M Bastiani D Sanchez (2000) ArticleTitleA phylogenetic analysis of the lipocalin protein family Mol Biol Evol 17 114–126 Occurrence Handle10666711 Occurrence Handle1:CAS:528:DC%2BD3cXot1SjsQ%3D%3D

    PubMed  CAS  Google Scholar 

  • J Garde SC Bell IC Eperon (1991) ArticleTitleMultiple forms of mRNA encoding human pregnancy-associated endometrial a2-globulin, a β-lactoglobulin homologue Proc Natl Acad Sci USA 88 2456–2460 Occurrence Handle2006183 Occurrence Handle1:CAS:528:DyaK3MXlt1yku7Y%3D

    PubMed  CAS  Google Scholar 

  • IR Horowitz C Cho M Song LC Flowers N Santanam S Parthasarathy S Ramachandran (2001) ArticleTitleIncreased glycodelin levels in gynaecological malignancies Int J Gynaecol Cancer 11 173–179 Occurrence Handle1:STN:280:DC%2BD3MzosVKmtQ%3D%3D

    CAS  Google Scholar 

  • ML Huhtala M Seppälä A Narvanen P Palomaki M Julkunen H Bohn (1987) ArticleTitleAmino acid sequence homology between human placental protein 14 and beta-lactoglobulins from various species Endocrinology 120 2620–2622 Occurrence Handle3569148 Occurrence Handle1:CAS:528:DyaL2sXksFCnsrs%3D

    PubMed  CAS  Google Scholar 

  • Jeschke U, Karsten U, Haase D (1999) Monoklonale Antikörper gegen Glycodelin A, Verfahren zu ihrer Herstellung und ihre Verwendung. Deutsches Patent- und Markenamt, Bundesdruckerei, Offenlegungsschrift: DE 198 07 389 A1.

  • M Julkunen R Koistinen J Sjöberg EM Rutanen T Wahlström M Seppälä (1986a) ArticleTitleSecretory endometrium synthesizes placental protein 14 Endocrinology 118 1782–1786 Occurrence Handle1:CAS:528:DyaL28Xit1ant7k%3D Occurrence Handle10.1210/endo-118-5-1782

    Article  CAS  Google Scholar 

  • M Julkunen R Koistinen AM Suikkari M Seppälä OA Janne (1990) ArticleTitleIdentification by hybridization histochemistry of human endometrial cells expressing mRNAs encoding a uterine beta-lactoglobulin homologue and insulin-like growth factor-binding protein-1 Mol Endocrinol 4 700–707 Occurrence Handle1703273 Occurrence Handle1:CAS:528:DyaK3cXkslWrs7k%3D

    PubMed  CAS  Google Scholar 

  • M Julkunen EM Rutanen A Koskimies T Ranta H Bohn M Seppälä (1985) ArticleTitleDistribution of placental protein 14 in tissues and body fluids during pregnancy Br J Obstet Gynaecol 92 1145–1151 Occurrence Handle4063232 Occurrence Handle1:CAS:528:DyaL28Xjt1Cisw%3D%3D

    PubMed  CAS  Google Scholar 

  • M Julkunen M Seppälä O Jänne (1988) ArticleTitleComplete amino acid sequence of human placental protein homologous to beta-lactoglobulin Proc Natl Acad Sci USA 85 8845–8849 Occurrence Handle3194393 Occurrence Handle1:CAS:528:DyaL1MXkvFWntr4%3D

    PubMed  CAS  Google Scholar 

  • M Julkunen T Wahlström M Seppälä (1986b) ArticleTitleHuman fallopian tube contains placental protein 14 Am J Obstet Gynaecol 154 1076–1079 Occurrence Handle1:CAS:528:DyaL28XktVCjs74%3D

    CAS  Google Scholar 

  • M Julkunen T Wahlström M Seppälä R Koistinen A Koskimies UH Stenman H Bohn (1984) ArticleTitleDetection and localization of placental protein 14-like protein in human seminal plasma and in the male genital tract Arch Androl 12 59–67 Occurrence Handle10.3109/01485018409161150 Occurrence Handle6398989

    Article  PubMed  Google Scholar 

  • M Kämäräinen M Halttunen R Koistinen K von Boguslawsky K von Smitten LC Andersson M Seppälä (1999) ArticleTitleExpression of glycodelin in human breast and breast cancer Int J Cancer 83 738–742 Occurrence Handle10597188

    PubMed  Google Scholar 

  • M Kämäräinen I Leivo M Julkunen M Seppälä (1993) ArticleTitleLocalization of progesterone-associated endometrial protein mRNA by in situ hybridization in human pregnancy decidua, endometriosis and borderline endometrioid adenoma J Mol Endocrinol 10 71–77 Occurrence Handle8452641

    PubMed  Google Scholar 

  • M Kämäräinen I Leivo R Koistinen M Julkunen U Karvonen EM Rutanen M Seppälä (1996) ArticleTitleNormal human ovary and ovarian tumors express glycodelin, a glycoprotein with immunosuppressive and contraceptive properties Am J Pathol 148 1435–1443 Occurrence Handle8623915

    PubMed  Google Scholar 

  • M Kämäräinen M Miettinen M Seppälä Boguslawsky K von MS Benassi T Bohling LC Andersson (1998) ArticleTitleEpithelial expression of glycodelin in biphasic synovial sarcomas Int J Cancer 76 487–490 Occurrence Handle9590122

    PubMed  Google Scholar 

  • M Kämäräinen L Riittinen M Seppälä A Palotie LC Andersson (1994) ArticleTitleProgesterone-associated endometrial protein (PAEP) - A constitutive marker of human erythroid precursors Blood 84 467–473 Occurrence Handle8025274

    PubMed  Google Scholar 

  • M Kämäräinen M Seppälä I Virtanen LC Andersson (1997) ArticleTitleExpression of glycodelin in MCF-7 breast cancer cells induces differentiation into organized acinar epithelium Lab Invest 77 565–573 Occurrence Handle9426393

    PubMed  Google Scholar 

  • Keil C (2000) Untersuchungen zur Lokalisation und Gensequenz des Glycodelins in Nicht-Primaten sowie zur Regulation der Glycodelin-Synthese in in vitro Modellen. Doctoral thesis, University of Greifswald.

  • C Keil B Husen J Giebel G Rune R Walther (1999) ArticleTitleGlycodelin mRNA is expressed in the genital tract of male and female rats (Rattus norvegicus) J Mol Endocrinol 23 57–66 Occurrence Handle10425447 Occurrence Handle1:CAS:528:DyaK1MXlt1Olt7k%3D

    PubMed  CAS  Google Scholar 

  • H Koistinen R Koistinen A Dell HR Morris RL Easton MS Patankar S Oehninger GF Clark M Seppälä (1996) ArticleTitleGlycodelin from seminal plasma is a differentially glycosylated form of contraceptive glycodelin-A Mol Hum Reprod 2 759–765 Occurrence Handle9239694 Occurrence Handle1:CAS:528:DyaK2sXosFar

    PubMed  CAS  Google Scholar 

  • H Koistinen R Koistinen C Hyden-Granskog O Magnus M Seppälä (2000) ArticleTitleSeminal plasma glycodelin and fertilization in vitro J Androl 21 636–640 Occurrence Handle10975409 Occurrence Handle1:CAS:528:DC%2BD3cXmsFSqsLg%3D

    PubMed  CAS  Google Scholar 

  • H Koistinen R Koistinen M Kamarainen J Salo M Seppala (1997) ArticleTitleMultiple forms of messenger ribonucleic acid encoding glycodelin in male genital tract Lab Invest 76 683–690 Occurrence Handle9166287 Occurrence Handle1:CAS:528:DyaK2sXjs1Sgs7Y%3D

    PubMed  CAS  Google Scholar 

  • PG Lalitkumar J Sengupta AA Karande D Ghosh (1998) ArticleTitlePlacental protein 14 in endometrium during menstrual cycle and effect of early luteal phase mifepristone administration on its expression in implantation stage endometrium in the rhesus monkey Hum Reprod 13 3478–3486 Occurrence Handle9886536 Occurrence Handle1:CAS:528:DyaK1MXltFOktA%3D%3D

    PubMed  CAS  Google Scholar 

  • HR Morris A Dell RL Easton M Panico H Koistinen R Koistinen S Oehninger MS Patankar M Seppälä GF Clark (1996) ArticleTitleGender-specific glycosylation of human glycodelin affects its contraceptive activity J Biol Chem 271 32159–32167 Occurrence Handle8943270 Occurrence Handle1:CAS:528:DyaK2sXit1SjtA%3D%3D

    PubMed  CAS  Google Scholar 

  • DM Morrow N Xiong RR Getty MZ Ratajczak D Morgan M Seppälä L Riittinen AM Gewirtz ML Tykocinski (1994) ArticleTitleHematopoietic placental protein 14: An immunosuppressive factor in cells of the megakaryocytic lineage Am J Pathol 145 1485–1495 Occurrence Handle7992851 Occurrence Handle1:STN:280:DyaK2M%2FosFSiug%3D%3D

    PubMed  CAS  Google Scholar 

  • S Oehninger CC Coddington GD Hodgen M Seppälä (1995) ArticleTitleFactors affecting fertilization: Endometrial placental protein 14 reduces the capacity of human spermatozoa to bind zona pellucida Fertil Steril 63 377–382 Occurrence Handle7531163 Occurrence Handle1:STN:280:DyaK2M7ksFOlsw%3D%3D

    PubMed  CAS  Google Scholar 

  • N Okamoto A Uchida K Takakura Y Kariya H Kanzaki L Riittinen R Koistinen M Seppälä T Mori (1991) ArticleTitleSuppression by human placental protein 14 of natural killer cell activity Am J Reprod Immunol 26 137–142 Occurrence Handle1840727 Occurrence Handle1:STN:280:DyaK3s%2FgtVCgsg%3D%3D

    PubMed  CAS  Google Scholar 

  • AG Pockley AE Bolton (1989) ArticleTitlePlacental protein 14 (PP14) inhibits the synthesis of interleukin-2 and the release of soluble interleukin-2 receptors from phytohaemagglutinin-stimulated lymphocytes Clin Exp Immunol 77 252–256 Occurrence Handle2789118 Occurrence Handle1:CAS:528:DyaL1MXlsFWkt7k%3D

    PubMed  CAS  Google Scholar 

  • AG Pockley AE Bolton (1990) ArticleTitleThe effect of human placental protein 14 (PP14) on the production of interleukin-1 from mitogenically stimulated mononuclear cell cultures Immunology 69 277–281 Occurrence Handle2307483 Occurrence Handle1:CAS:528:DyaK3cXhsFKns78%3D

    PubMed  CAS  Google Scholar 

  • AS Poddar JG Kim KP Gill BN Bates N Santanam JA Rock AA Murphy S Parthasarathy (1998) ArticleTitleGeneration and characterization of a polyclonal antipeptide antibody to human glycodelin Fertil Steril 69 543–548 Occurrence Handle9531894 Occurrence Handle1:STN:280:DyaK1c7ptlSntw%3D%3D

    PubMed  CAS  Google Scholar 

  • L Riittinen (1992) ArticleTitleSerous ovarian cyst fluids contain high levels of endometrial placental protein 14 Tumor Biol 13 175–179 Occurrence Handle1:STN:280:DyaK38zislGjsQ%3D%3D Occurrence Handle10.1159/000217762

    Article  CAS  Google Scholar 

  • L Riittinen O Narvanen I Virtanen M Seppala (1991) ArticleTitleMonoclonal antibodies against endometrial protein PP14 and their use for purification and radioimmunoassay of PP14 J. Immunol Methods 136 85–90 Occurrence Handle1995715 Occurrence Handle1:CAS:528:DyaK3MXltlWhs7Y%3D

    PubMed  CAS  Google Scholar 

  • L Riittinen UH Stenman H Alfthan AM Suikkari H Bohn M Seppälä (1989) ArticleTitleTime-resolved immunofluorometric assay for placental protein 14 Clin Chim Acta 183 115–123 Occurrence Handle2676243 Occurrence Handle1:CAS:528:DyaL1MXlslSnu78%3D

    PubMed  CAS  Google Scholar 

  • ED Ryan WH Walker (1980) ArticleTitleInfluence of tissue homogenization techniques on levels of estrogen and progesterone receptors measured in calf uterus and human breast tumors J. Immunoassay 1 463–474 Occurrence Handle7197689 Occurrence Handle1:CAS:528:DyaL3MXktlyls70%3D

    PubMed  CAS  Google Scholar 

  • E Saridogan O Djahanbakhch ME Kervancioglu F Kahyaoglu K Shrimanker JG Grudzinskas (1997) ArticleTitlePlacental protein 14 production by human Fallopian tube epithelial cells in vitro Hum Reprod 12 1500–1507 Occurrence Handle9262286 Occurrence Handle1:CAS:528:DyaK2sXls1Sgur0%3D

    PubMed  CAS  Google Scholar 

  • M Seppälä H Bohn Y Tatarinov (1998) ArticleTitleGlycodelins Tumor Biol. 19 213–220

    Google Scholar 

  • M Seppälä AI Koskimies A Tenhunen EM Rutanen J Sjoberg R Koistinen M Julkunen T Wahlstrom (1985) ArticleTitlePregnancy proteins in seminal plasma, seminal vesicles, preovulatory follicular fluid, and ovary Ann N Y Acad Sci 442 212–226 Occurrence Handle3893267

    PubMed  Google Scholar 

  • M Seppälä L Riittinen M Julkunen R Koistinen T Wahlstrom K Iino H Alfthan UH Stenman ML Huhtala (1988) ArticleTitleStructural studies, localization in tissue and clinical aspects of human endometrial proteins J Reprod Fertil Suppl 36 127–141 Occurrence Handle3057195

    PubMed  Google Scholar 

  • PK Smith RI Krohn GT Hermanson AK Mallia FH Gartner MD Provenzano EK Fujimoto NM Goeke BJ Olson DC Klenk (1985) ArticleTitleMeasurement of protein using bicinchoninic acid Anal Biochem 150 76–85 Occurrence Handle3843705 Occurrence Handle1:CAS:528:DyaL2MXlsFKksL0%3D

    PubMed  CAS  Google Scholar 

  • M Song S Ramaswamy S Ramachandran LC Flowers IR Horowitz JA Rock S Parthasarathy (2001) ArticleTitleAngiogenic role for glycodelin in tumorigenesis Proc Natl Acad Sci USA 98 9265–9270 Occurrence Handle11459932 Occurrence Handle1:CAS:528:DC%2BD3MXlvFSrs7g%3D

    PubMed  CAS  Google Scholar 

  • YuS Tatarinov LA Shcherbakova GA Olefirenko DD Petrunin (1990) ArticleTitleProtein factor of fertility in malignancies Tumor Biol 11 113–119 Occurrence Handle10.1159/000217645

    Article  Google Scholar 

  • RN Taylor JF Savouret C Vaisse JL Vigne I Ryan D Hornung M Seppälä E Milgrom (1998) ArticleTitlePromegestone (R5020) and mifepristone (RU486) both function as progestational agonists of human glycodelin gene expression in isolated human epithelial cells J Clin Endocrinol Metab 83 4006–4012 Occurrence Handle9814484 Occurrence Handle1:CAS:528:DyaK1cXntlOitLs%3D

    PubMed  CAS  Google Scholar 

  • JY Tse PC Chiu KF Lee M Seppälä H Koistinen R Koistinen YQ Yao WS Yeung (2002) ArticleTitleThe synthesis and fate of glycodelin in human ovary during folliculogenesis Mol Hum Reprod 8 142–148 Occurrence Handle11818517 Occurrence Handle1:CAS:528:DC%2BD38XitFOgsr0%3D

    PubMed  CAS  Google Scholar 

  • C Vaisse M Atger B Potier E Milgrom (1990) ArticleTitleHuman placental protein 14 gene: sequence and characterization of a short duplication DNA Cell Biol 9 401–413 Occurrence Handle2206398 Occurrence Handle1:CAS:528:DyaK3cXmtVKiurY%3D

    PubMed  CAS  Google Scholar 

  • N Van Cong C Vaisse MS Gross R Slim E Milgrom A Bernheim (1991) ArticleTitleThe human placental protein 14 (PP14) gene is localized on chromosome 9q34 Hum Genet 86 515–518 Occurrence Handle2016092 Occurrence Handle1:STN:280:DyaK3M3gtVyitw%3D%3D

    PubMed  CAS  Google Scholar 

  • GT Waites RF James RA Walker SC Bell (1990) ArticleTitleHuman ‘pregnancy-associated endometrial alpha 1-globulin, a 32 kDa insulin-like growth factor-binding protein: immunohistological distribution and localization in the adult and fetus J Endocrinol 124 333–339 Occurrence Handle2313221 Occurrence Handle1:CAS:528:DyaK3cXpsF2ltw%3D%3D Occurrence Handle10.1677/joe.0.1240333

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Pathophysiology, Ernst Moritz Arndt University, Greifswalder Street 11C, 17495, Karlsburg, Germany

    Christiane Kunert-Keil

  2. Institute of Anatomy, Ernst Moritz Arndt University, Friedrich Loeffler Street 23C, 17487, Greifswald, Germany

    Esther Wiehmeier & Jürgen Giebel

  3. Department of Obstetrics and Gynaecology, Ludwig Maximilians University of Munich, Maistrasse 11, 80337, Munich, Germany

    Udo Jeschke

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  1. Christiane Kunert-Keil
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  2. Esther Wiehmeier
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  3. Udo Jeschke
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  4. Jürgen Giebel
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Kunert-Keil, C., Wiehmeier, E., Jeschke, U. et al. Immunolocalization of glycodelin in the genital tract of rats. J Mol Hist 36, 111–117 (2005). https://doi.org/10.1007/s10735-004-4272-1

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  • DOI: https://doi.org/10.1007/s10735-004-4272-1

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