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Nuclear myosin is ubiquitously expressed and evolutionary conserved in vertebrates

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Abstract

Nuclear myosin I (NMI) is a single-headed member of myosin superfamily localized in the cell nucleus which participates along with nuclear actin in transcription and chromatin remodeling. We demonstrate that NMI is present in cell nuclei of all mouse tissues examined except for cells in terminal stages of spermiogenesis. Quantitative PCR and western blots demonstrate that the expression of NMI in tissues varies with the highest levels in the lungs. The expression of NMI is lower in serum-starved cells and it increases after serum stimulation. The lifespan of NMI is longer than 16 h as determined by cycloheximide translation block. A homologous protein is expressed in human, chicken, Xenopus, and zebrafish as shown by RACE analysis. The analysis of genomic sequences indicates that almost identical homologous NMI genes are expressed in mammals, and similar NMI genes in vertebrates.

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Acknowledgments

We are grateful to Peter G. Gillespie for the anti-Myo1c antibody, Lenka Rossmeislová and Zdeněk Hodný for critically reading the manuscript. This work was supported by the Grant Agency of Czech Republic (reg. no. 204/04/0108), Grant Agency of the Academy of Sciences of the Czech Republic (reg. No. IAA5039202), grant LC545 of the MSMT, and by the institutional grant no. AV0Z50390512. MŠ and RD were supported by the student program of the Grant Agency of the Czech Republic (reg. No. 204/05/H023 IV).

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Authors and Affiliations

  1. Institute of Experimental Medicine, Department of Cell Ultrastructure and Molecular Biology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague 4, Czech Republic

    M. Kahle, J. Přidalová, M. Špaček, R. Dzijak & P. Hozák

  2. Institute of Molecular Genetics, Department of Biology of the Cell Nucleus, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague 4, Czech Republic

    M. Kahle & P. Hozák

  3. Department of Histology and Embryology, 3rd Faculty of Medicine, Charles University, Ruská 87, 100 00, Prague 10, Czech Republic

    M. Špaček

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  1. M. Kahle
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  2. J. Přidalová
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  3. M. Špaček
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  4. R. Dzijak
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  5. P. Hozák
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Correspondence to P. Hozák.

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Kahle, M., Přidalová, J., Špaček, M. et al. Nuclear myosin is ubiquitously expressed and evolutionary conserved in vertebrates. Histochem Cell Biol 127, 139–148 (2007). https://doi.org/10.1007/s00418-006-0231-0

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